Kütan Esma, Duygu-Çapar Gonca, Özçakir-Tomruk Ceyda, Dilek Ozkan Cem, Özen Fatma, Erdoğan Özge, Özdemir Ipek, Korachi May, Gürel Aydin
Department of Oral Implantology, Faculty of Dentistry, Yeditepe University,Göztepe, Istanbul, Turkey.
Department of Oral and Maxillofacial Surgery, Trakya University, Edirne, Turkey.
Arch Oral Biol. 2016 Mar;63:15-21. doi: 10.1016/j.archoralbio.2015.11.001. Epub 2015 Nov 23.
The effects of systemic antibiotics on controlling infective pathogens after guided bone regeneration(GBR) procedures especially in membrane exposures are limited. However, local administrations of antibiotics are rare in GBR techniques.
The aim of this study was to investigate the osteogenesis potential and the antibacterial effect of a doxycycline releasing collagen membrane in surgically created and contaminated defects in rat tibiae.
Defects were created in 20 rats that were randomly divided in to two groups: control group (defect contaminated by Porphyromonas gingivalis, filled with bone graft and covered by collagen membrane); test group (defect contaminated by P. gingivalis filled with bone graft and covered by collagen membrane containing 1mg/cm(2) doxycycline. Animals were sacrificed post surgically on the 14th day for microbiologic evaluation and on the 28th day for histopathological evaluation.
The degree of osteogenesis in the test group was seen to be significantly higher than control group (p: 0.011; p<0.05). Furthermore in test group, no bacterial growth was observed. The bacteria counts were determined between 1×104 and 268×104CFU/g with a median of 1.32×104 for control group.
Within the limitations of this study, the results of the present study suggests that the use of a doxycycline releasing membrane has a positive effect on contaminated GBR procedures for limiting P. gingivalis infections leading to bone formation following GBR procedures in a rat model.
全身使用抗生素对引导骨再生（GBR）术后控制感染病原体的效果有限，尤其是在膜暴露的情况下。然而，在GBR技术中局部使用抗生素的情况很少见。
本研究旨在探讨在大鼠胫骨手术造成的污染性骨缺损中，释放强力霉素的胶原膜的成骨潜力和抗菌效果。
在20只大鼠身上制造骨缺损，随机分为两组：对照组（骨缺损被牙龈卟啉单胞菌污染，填充骨移植材料并用胶原膜覆盖）；试验组（骨缺损被牙龈卟啉单胞菌污染，填充骨移植材料并用含1mg/cm²强力霉素的胶原膜覆盖）。术后第14天处死动物进行微生物学评估，第28天处死进行组织病理学评估。
试验组成骨程度明显高于对照组（p：0.011；p<0.05）。此外，试验组未观察到细菌生长。对照组细菌计数在1×10⁴至268×10⁴CFU/g之间，中位数为1.32×10⁴。
在本研究的局限性范围内，本研究结果表明，在大鼠模型中，使用释放强力霉素的膜对受污染的GBR手术具有积极作用，可限制牙龈卟啉单胞菌感染，从而促进GBR手术后的骨形成。
