Department of Endodontics, Araçatuba School of Dentistry, Univ Estadual Paulista, Araçatuba, SP, Brazil.
Clin Oral Implants Res. 2012 Mar;23(3):384-8. doi: 10.1111/j.1600-0501.2011.02166.x. Epub 2011 Mar 28.
The aim of the present investigation was to histologically analyze the effect of using lyophilized bovine bone (GenOx® organic matrix) with (or without) guided tissue regeneration (using a decalcified cortical osseous membrane [GenDerm®]) on bone healing in surgically created critical-size defects created in rat tibia.
Surgical critical-size bone defects were created in 64 animals that were randomly divided into four groups: group I (control); group II (defect filled with GenOx®); group III (defect covered by GenDerm®); group IV (defect filled with GenOx® and covered by GenDerm®). Animals were killed at 30 or 90 days post-surgery. The specimens were embedded in paraffin, serially cut, and stained with hematoxylin and eosin for analysis under light microscopy. The formation of new bone in the cortical area of the defect was histomorphometrically evaluated.
All experimental groups demonstrated superior bone healing compared with the control group. However, group IV samples showed evidence of more advanced healing at both 30 and 90 days post-surgery as compared with the other experimental groups.
The bovine organic bone graft GenOx® associated with GenDerm® this produced the best treatment results in the case of critical-size defects in rat tibia.
本研究旨在通过组织学分析，探讨冻干牛骨（GenOx®有机基质）联合（或不联合）引导组织再生（使用脱钙皮质骨膜[GenDerm®]）对大鼠胫骨外科造模临界尺寸骨缺损中骨愈合的影响。
64 只动物被随机分为 4 组：I 组（对照组）；II 组（用 GenOx®填充缺损）；III 组（用 GenDerm®覆盖缺损）；IV 组（用 GenOx®填充缺损并用 GenDerm®覆盖）。术后 30 或 90 天处死动物。标本行石蜡包埋、连续切片、苏木精-伊红染色，在光镜下进行分析。对皮质区缺损内新骨形成进行组织形态计量学评估。
与对照组相比，所有实验组的骨愈合均得到改善。然而，与其他实验组相比，在术后 30 天和 90 天，IV 组的样本显示出更先进的愈合迹象。
在大鼠胫骨临界尺寸缺损的情况下，冻干牛骨移植物 GenOx®联合 GenDerm®的治疗效果最佳。
