Ribeiro Luciana Liarte Gasparini, Bosco Alvaro Francisco, Nagata Maria José Hitomi, de Melo Luiz Gustavo Nascimento
Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, University of State of São Paulo "Júlio de Mesquita Filho"-UNESP, Araçatuba, São Paulo, Brazil.
Int J Oral Maxillofac Implants. 2008 Sep-Oct;23(5):811-7.
The purpose of this study was to histologically analyze the influence of bioactive glass and/or acellular dermal matrix on bone healing in surgically created defects in the tibiae of 64 rats (Rattus norvegicus, albinus, Wistar).
A 4-mm x 3-mm unicortical defect was created on the anterolateral surface of the tibia. Animals were divided into 4 groups: C, control; BG, the defect was filled with bioactive glass; ADM, the defect was covered with acellular dermal matrix; and BG/ADM, the defect was filled with bioactive glass and covered with acellular dermal matrix. Animals were sacrificed at 10 or 30 days postoperatively, and the specimens were removed for histologic processing. The formation of new bone in the cortical area of the defect was evaluated histomorphometrically.
At 10 and 30 days postoperatively, groups C (39.65% +/- 5.63%/63.34% +/- 5.22%) and ADM (38.12% +/- 5.53/58.96% +/- 7.05%) presented a larger amount of bone formation compared to the other groups (P < .05). In the same periods, groups BG (13.10% +/- 6.29%/29.5% +/- 5.56%) and BG/ADM (20.72% +/- 8.31%/24.19% +/- 6.69%) exhibited statistically similar new bone formation. However, unlike the other groups, group BG/ADM did not present a significant increase in bone formation between the 2 time points.
Based on these results, it can be concluded that all of the materials used in this study delayed bone healing in non-critical-size defects.
本研究旨在通过组织学分析生物活性玻璃和/或脱细胞真皮基质对64只大鼠（挪威大鼠、白化大鼠、Wistar大鼠）胫骨手术造成的缺损处骨愈合的影响。
在胫骨前外侧表面制造一个4毫米×3毫米的单皮质缺损。将动物分为4组：C组为对照组；BG组，缺损处填充生物活性玻璃；ADM组，缺损处覆盖脱细胞真皮基质；BG/ADM组，缺损处填充生物活性玻璃并覆盖脱细胞真皮基质。术后10天或30天处死动物，取出标本进行组织学处理。通过组织形态计量学评估缺损皮质区域新骨的形成。
术后10天和30天，C组（39.65%±5.63%/63.34%±5.22%）和ADM组（38.12%±5.53/58.96%±7.05%）与其他组相比，骨形成量更多（P<.05）。在同一时期，BG组（13.10%±6.29%/29.5%±5.56%）和BG/ADM组（20.72%±8.31%/24.19%±6.69%）的新骨形成在统计学上相似。然而，与其他组不同的是，BG/ADM组在两个时间点之间骨形成没有显著增加。
基于这些结果，可以得出结论，本研究中使用的所有材料均延迟了非临界尺寸缺损处的骨愈合。
