孤立性低级别尿路上皮癌患者中肿瘤相关巨噬细胞和自然杀伤细胞与膀胱癌大小及T分期的相关性
Correlation of tumor-associated macrophages and NK cells with bladder cancer size and T stage in patients with solitary low-grade urothelial carcinoma.
作者信息
Krpina Kristian, Babarović Emina, Španjol Josip, Đorđević Gordana, Maurer Tobias, Jonjić Nives
机构信息
Department of Urology, Clinical Hospital Center Rijeka, Tome Strižića 3, 51000, Rijeka, Croatia.
Department of Pathology, School of Medicine, University of Rijeka, Braće Branchetta 20, 51000, Rijeka, Croatia.
出版信息
Wien Klin Wochenschr. 2016 Apr;128(7-8):248-52. doi: 10.1007/s00508-015-0907-3. Epub 2015 Dec 11.
OBJECTIVE
The aim of the present study was to correlate the level of tumor-infiltrating immune cells with bladder cancer size and T category in patients with solitary low-grade non-muscle invasive bladder cancer (NMIBC).
PATIENTS AND METHODS
Between 1996 and 2006, 115 patients with solitary low-grade NMIBC after transurethral resection of the bladder without adjuvant therapy were retrospectively identified from the institutional database. Tumor specimens were retrieved and tissue microarrays were constructed. Immunhistochemical staining for tumor-infiltrating immune cells with anti-CD3, CD4, CD8, CD20, CD56, CD68, and granzyme B (Gr B) was performed.
RESULTS
Immune cells were predominantly observed within the cancer stroma. Statistically significant higher levels of CD56 cells in small tumors and CD68 cells in T1 tumors (p = 0.0310, 0.0151, respectively) were established.
CONCLUSION
The current study propose a possible correlation of CD56+ and CD68+ cells with bladder cancer size and stage in patients with solitary low-grade NMIBC.
目的
本研究旨在探讨孤立性低级别非肌层浸润性膀胱癌（NMIBC）患者肿瘤浸润免疫细胞水平与肿瘤大小及T分期的相关性。
患者与方法
回顾性分析1996年至2006年间，115例经尿道膀胱肿瘤电切术后未接受辅助治疗的孤立性低级别NMIBC患者资料，所有患者资料均来自机构数据库。收集肿瘤标本并构建组织芯片，采用抗CD3、CD4、CD8、CD20、CD56、CD68及颗粒酶B（Gr B）抗体对肿瘤浸润免疫细胞进行免疫组化染色。
结果
免疫细胞主要浸润于癌间质。小肿瘤中CD56细胞水平及T1期肿瘤中CD68细胞水平均显著升高（p值分别为0.0310、0.0151）。
结论
本研究表明，孤立性低级别NMIBC患者中，CD56+和CD68+细胞水平可能与肿瘤大小及分期相关。
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