Azuma Kagaku, Furuzawa Manabu, Fujiwara Shu, Yamada Kumiko, Kubo Kin-ya
1. Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, Fukuoka, 807-8555, Japan.
2. Department of Prosthodontics, Asahi University School of Dentistry, Mizuho, 501-0296, Japan.
Int J Med Sci. 2015 Nov 4;12(12):952-7. doi: 10.7150/ijms.13298. eCollection 2015.
Chronic psychologic stress increases corticosterone levels, which decreases bone density. Active mastication or chewing attenuates stress-induced increases in corticosterone. We evaluated whether active mastication attenuates chronic stress-induced bone loss in mice. Male C57BL/6 (B6) mice were randomly divided into control, stress, and stress/chewing groups. Stress was induced by placing mice in a ventilated restraint tube (60 min, 2x/day, 4 weeks). The stress/chewing group was given a wooden stick to chew during the experimental period. Quantitative micro-computed tomography, histologic analysis, and biochemical markers were used to evaluate the bone response. The stress/chewing group exhibited significantly attenuated stress-induced increases in serum corticosterone levels, suppressed bone formation, enhanced bone resorption, and decreased trabecular bone mass in the vertebrae and distal femurs, compared with mice in the stress group. Active mastication during exposure to chronic stress alleviated chronic stress-induced bone density loss in B6 mice. Active mastication during chronic psychologic stress may thus be an effective strategy to prevent and/or treat chronic stress-related osteopenia.
慢性心理应激会增加皮质酮水平，进而降低骨密度。主动咀嚼可减轻应激诱导的皮质酮升高。我们评估了主动咀嚼是否能减轻小鼠慢性应激诱导的骨质流失。将雄性C57BL/6（B6）小鼠随机分为对照组、应激组和应激/咀嚼组。通过将小鼠置于通风的束缚管中诱导应激（60分钟，每天2次，共4周）。在实验期间，给应激/咀嚼组一根木棍供其咀嚼。采用定量微计算机断层扫描、组织学分析和生化标志物来评估骨骼反应。与应激组小鼠相比，应激/咀嚼组血清皮质酮水平应激诱导的升高明显减轻，骨形成受到抑制，骨吸收增强，椎骨和股骨远端的小梁骨量减少。在慢性应激暴露期间主动咀嚼可减轻B6小鼠慢性应激诱导的骨密度损失。因此，在慢性心理应激期间主动咀嚼可能是预防和/或治疗慢性应激相关骨质减少的有效策略。
