Reuter Raphael, Ward Thomas R
Department of Chemistry, University of Basel, Spitalstrasse 51, CH-4056 Basel, Switzerland.
Beilstein J Org Chem. 2015 Oct 12;11:1886-92. doi: 10.3762/bjoc.11.203. eCollection 2015.
Herein we report on a 96-well plate assay based on the fluorescence resulting from the ring-closing metathesis of two profluorophoric substrates. To demonstrate the validity of the approach, four commercially available ruthenium-metathesis catalysts were evaluated in six different solvents. The results from the fluorescent assay agree well with HPLC conversions, validating the usefulness of the approach.
在此，我们报道了一种基于两种前荧光底物闭环复分解反应产生的荧光的96孔板检测方法。为了证明该方法的有效性，在六种不同溶剂中评估了四种市售的钌复分解催化剂。荧光检测结果与高效液相色谱转化率非常吻合，验证了该方法的实用性。
