Farkaš Pavol, Čížová Alžbeta, Bystrický Peter, Paulovičová Lucia, Paulovičová Ema, Bystrický Slavomír
Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic.
Glycoconj J. 2016 Feb;33(1):113-20. doi: 10.1007/s10719-015-9644-0. Epub 2015 Dec 14.
An efficient method for preparation of fluorescently labelled mannan-peptide glycoconjugates has been developed. After selective Dess-Martin periodinane oxidation of mannan, it was conjugated to the fluorescent label alone and a peptide with the label via reductive amination. Prepared glycoconjugates were characterised by HPSEC, FTIR-ATR and UV-VIS spectroscopy. Finally, the fluorescently labelled mannan and mannan-peptide conjugate were used for microscopic visualization of their accumulation in intracellular organelles of RAW 264.7 cells.
