Carbajal Mejía Jeison B, Aguilar Arrieta Angela
Department of Endodontics, Faculty of Dentistry, Daniel Alcides Carrión National University, UNDAC, Pasco, Peru.
Department of Fixed Prosthodontics, Osaka University Graduate School of Dentistry, Suita, Japan.
Dent Traumatol. 2016 Aug;32(4):321-7. doi: 10.1111/edt.12250. Epub 2015 Dec 15.
The purpose of this study was to evaluate the viability of E. faecalis after a 14-day exposure to 1% cetrimide (CET), triantibiotic paste (i.e., metronidazole, minocycline, and ciprofloxacin), 2% chlorhexidine (CHX) gel, and calcium hydroxide (Ca[OH]2 ) in an infected dentine model.
A total of 75 roots of extracted uniradicular human teeth were chemomechanically prepared, sterilized, and infected for 21 days with E. faecalis. Samples were divided into five groups (n = 15) to apply intracanal medicaments, namely saline solution (negative control), 1% CET, triantibiotic paste (TRIA), 2% CHX gel, and Ca(OH)2 during 14 days. Dentine samples were collected and stained with the SYTO 9/propidium iodide technique (Live/Dead, Bacligth, Invitrogen, Eugene, OR, USA) for fluorescence microscopy to obtain the percentage of viable cells. Statistical analysis was performed using one-way analysis of variance followed by Tukey's multiple comparison test (P < 0.05).
All experimental agents significantly reduced the percentage of viable E. faecalis when compared with the control group. In addition, the least cytotoxic medicament was Ca(OH)2 followed by CHX. There was no significant difference between 1% CET and TRIA (P = 0.98).
Both 1% CET and TRIA significantly reduced the viability of E. faecalis in dentine of extracted teeth in comparison with 2% CHX gel and calcium hydroxide paste. Further laboratory and clinical investigations should be carried out to validate findings of the beneficial use of 1% CET as an intracanal medicament against E. faecalis.
本研究的目的是在感染牙本质模型中评估粪肠球菌在暴露于1%西曲溴铵（CET）、三联抗生素糊剂（即甲硝唑、米诺环素和环丙沙星）、2%氯己定（CHX）凝胶和氢氧化钙（Ca[OH]₂）14天后的生存能力。
总共75颗拔除的单根人牙进行化学机械预备、消毒，并感染粪肠球菌21天。将样本分为五组（n = 15），在14天内分别应用根管内药物，即生理盐水（阴性对照）、1% CET、三联抗生素糊剂（TRIA）、2% CHX凝胶和Ca(OH)₂。收集牙本质样本，并用SYTO 9/碘化丙啶技术（死活细胞染色试剂盒，Bacligth，美国俄勒冈州尤金市英杰公司）染色，用于荧光显微镜观察，以获得活细胞百分比。采用单因素方差分析，随后进行Tukey多重比较检验进行统计分析（P < 0.05）。
与对照组相比，所有实验药物均显著降低了粪肠球菌的存活百分比。此外，细胞毒性最小的药物是Ca(OH)₂，其次是CHX。1% CET和TRIA之间无显著差异（P = 0.98）。
与2% CHX凝胶和氢氧化钙糊剂相比，1% CET和TRIA均显著降低了拔除牙牙本质中粪肠球菌的生存能力。应进一步开展实验室和临床研究，以验证1% CET作为根管内药物对抗粪肠球菌有益作用的研究结果。
