Charan Teja Garapati Venkata, Nandana Raju Mahali Raghu, Neelima Reddy Uppu Lavanya, V Satyanarayana Uppalapati V, Praneeth Devatha, Maheswari Kumpatla
Department of Conservative Dentistry and Endodontics, Lenora Institute of Dental Sciences, Rajahmundry, IND.
Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, IND.
Cureus. 2022 Jun 30;14(6):e26455. doi: 10.7759/cureus.26455. eCollection 2022 Jun.
Despite scientific evidence that even some microorganisms may be useful, endodontic intervention has persisted to prioritize the removal of all microorganisms from the root canal system. Indeed, information regarding the significant role of probiotic microorganisms in endodontic treatment has been sparse. This study aimed to carry out an in vitro evaluation of the antimicrobial activity of probiotics against endodontic pathogens.
The evaluation was carried out in three stages. In Stage 1, the agar cup well procedure was used to analyse the efficiency of probiotics microorganisms against bacteria and microorganisms in the planktonic stage. In Stage 2, a deferred antagonistic experiment was used to determine the activity of probiotic microorganisms against endodontic pathogens like and in the planktonic phase. In Stage 3, biofilm phase evaluation of an intracanal probiotic microorganism carrier was done. The region of maximum inhibition was measured at the end of Stages 1 and 2. The antimicrobial activity was recognized when the dimension of the region of maximum inhibition was 10 mm or above. The colony-forming unit/millilitre was measured at the end of Stage 3.
There was marked antimicrobial activity of probiotic microorganisms against the pathogenic microorganisms as well as in Stages 1 and 3, i.e., during the evaluation involving agar cup and evaluation at the biofilm stage. However, no antimicrobial activity of probiotic microorganisms was observed against pathogenic endodontic microorganisms in Stage 2, i.e., during evaluation involving the use of the deferred antagonistic technique.
It can be concluded that probiotic therapy is a promising antibacterial treatment approach that should be further investigated. This study shows that probiotics can help effectively in endodontic treatment and that more in vitro as well as in vivo research is needed to fully appreciate the advantages of bacteriotherapy in the field of endodontics.
尽管有科学证据表明即使某些微生物可能有益，但牙髓治疗一直坚持将从根管系统中清除所有微生物作为首要任务。事实上，关于益生菌微生物在牙髓治疗中的重要作用的信息一直很少。本研究旨在对益生菌对牙髓病原体的抗菌活性进行体外评估。
评估分三个阶段进行。在第1阶段，采用琼脂杯孔法分析益生菌微生物对浮游阶段细菌和微生物的有效性。在第2阶段，采用延迟拮抗实验来确定益生菌微生物对浮游阶段牙髓病原体如[具体病原体1]和[具体病原体2]的活性。在第3阶段，对根管内益生菌微生物载体进行生物膜阶段评估。在第1阶段和第2阶段结束时测量最大抑制区域。当最大抑制区域的尺寸为10毫米或以上时，确认具有抗菌活性。在第3阶段结束时测量每毫升菌落形成单位。
在第1阶段和第3阶段，即涉及琼脂杯评估和生物膜阶段评估期间，益生菌微生物对致病微生物[具体病原体1]和[具体病原体2]具有显著的抗菌活性。然而，在第2阶段，即在涉及使用延迟拮抗技术的评估期间，未观察到益生菌微生物对致病性牙髓微生物的抗菌活性。
可以得出结论，益生菌疗法是一种有前途的抗菌治疗方法，应进一步研究。本研究表明，益生菌可以在牙髓治疗中有效发挥作用，并且需要更多的体外和体内研究来充分认识细菌疗法在牙髓病学领域的优势。
