Jain Gaurav, Singh Priyansha, Agarwal Kishan
Department of Conservative Dentistry and Endodontics, Saraswati Dental College, Lucknow, Uttar Pradesh, India.
J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1538-S1543. doi: 10.4103/jpbs.jpbs_277_21. Epub 2021 Nov 10.
is the most common and important microorganism found in infected root canals associated with persistent periapical periodontitis and failing endodontically treated tooth. Intracanal medicaments used after chemomechanical preparation of an infected root canal play a vital in eradication of this microorganism and pave the way for long-term success of endodontic therapy. Hence, the present study was conducted to assess the antimicrobial efficacy of calcium hydroxide (Ca(OH)), triple antibiotic paste (metronidazole 400 mg + minocycline 100 mg + ciprofloxacin 500 mg), and 2% chlorhexidine (CHX) combined with 0.5% cetrimide on eradication of biofilm.
Eighty dentin specimens were taken and infected extraorally with to induce microbial colonization. The specimens were then divided into four groups of twenty each based on medicaments used and further subdivided into two subgroups based on assessment of live cells done either immediately after the elimination of the medicament or after 24-h incubation in brain-heart infusion (BHI) medium: Group I specimens were treated with Ca(OH), Group II with triple antibiotic paste, Group III with 2% CHX combined with 0.5% cetrimide, and Group IV with saline (control) for 7 days at 37°C. Assessment of live cells was done using confocal microscope.
2% CHX combined with 0.5% cetrimide (Group III) and triple antibiotic paste (Group II) showed a statistically significant result with high antimicrobial efficacy and lower percentage of live cells as compared to Ca(OH) (Group I). The mean percentage of live cells in Group I immediately after elimination of medicaments was 64.7%, in Group II was 1.52%, in Group III was 1.49%, and in Group IV was 83.4%. After 24 h of incubation in BHI medium, 2% CHX combined with 0.5% cetrimide (Group III) showed a statistically significant ( < 0.05) result of 1.27% mean live cells as compared to 84.2% in Ca(OH) (Group I), 1.82% in triple antibiotic paste (Group II), and 94.2% in saline (Group IV control).
2% CHX combined with 0.5% cetrimide exhibited maximum antimicrobial efficacy with least number of mean live cells followed by triple antibiotic paste as compared to Ca(OH). Based on these findings, 2% CHX combined with 0.5% cetrimide was most effective in eradicating from the extraorally infected dentine biofilm.
是在与持续性根尖周炎和根管治疗失败的牙齿相关的感染根管中发现的最常见且最重要的微生物。在对感染根管进行化学机械预备后使用的根管内药物对于根除这种微生物起着至关重要的作用，并为根管治疗的长期成功铺平道路。因此，本研究旨在评估氢氧化钙（Ca(OH)）、三联抗生素糊剂（甲硝唑400毫克+米诺环素100毫克+环丙沙星500毫克）以及2%氯己定（CHX）与0.5%西曲溴铵联合使用对生物膜根除的抗菌效果。
采集80个牙本质标本，在口外感染以诱导微生物定植。然后根据所使用的药物将标本分为四组，每组20个，并根据在去除药物后立即或在脑心浸液（BHI）培养基中孵育24小时后对活细胞进行的评估进一步细分为两个亚组：第一组标本用Ca(OH)处理，第二组用三联抗生素糊剂处理，第三组用2%CHX与0.5%西曲溴铵联合处理，第四组用生理盐水（对照组）在37°C下处理7天。使用共聚焦显微镜对活细胞进行评估。
与Ca(OH)（第一组）相比，2%CHX与0.5%西曲溴铵联合使用（第三组）和三联抗生素糊剂（第二组）显示出具有统计学意义的结果，抗菌效果高且活细胞百分比更低。在去除药物后立即，第一组活细胞的平均百分比为64.7%，第二组为1.52%，第三组为1.49%，第四组为83.4%。在BHI培养基中孵育24小时后，与Ca(OH)（第一组）中的84.2%、三联抗生素糊剂（第二组）中的1.82%以及生理盐水（第四组对照组）中的94.2%相比，2%CHX与0.5%西曲溴铵联合使用（第三组）显示出平均活细胞为1.27%的具有统计学意义（P<0.05）的结果。
与Ca(OH)相比，2%CHX与0.5%西曲溴铵联合使用表现出最大的抗菌效果，平均活细胞数量最少，其次是三联抗生素糊剂。基于这些发现，2%CHX与0.5%西曲溴铵联合使用在根除口外感染的牙本质生物膜中的方面最有效。
