School of Mechanical Engineering, University of Birmingham, Edgbaston, UK.
J Tissue Eng. 2012;3(1):2041731412454672. doi: 10.1177/2041731412454672. Epub 2012 Jul 9.
In this study, the effect of pulsed-low intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by 3T3 fibroblasts encapsulated in alginate was evaluated. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content and dimethylamine blue assay for glycosaminoglycan content were performed on samples from cell cultures treated with pulsed-low intensity ultrasound and a control group. Pulsed-low intensity ultrasound shows no effect on cell proliferation, while collagen and glycosaminoglycan contents were consistently higher in the samples treated with pulsed-low intensity ultrasound, showing a statistically significant difference (p < 0.05) on day 10. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both groups. These results suggest that pulsed-low intensity ultrasound shows no effect on cell proliferation but has potential for inducing collagen and glycosaminoglycan production in cells cultured in alginate gels.
本研究评估了脉冲低强度超声对藻酸盐包埋的 3T3 成纤维细胞增殖、胶原产生和糖胺聚糖沉积的影响。用 Hoechst 33258 法检测细胞数量，羟脯氨酸法检测胶原含量，二甲胺蓝法检测糖胺聚糖含量，对经脉冲低强度超声处理和对照组的细胞培养物样本进行检测。脉冲低强度超声对细胞增殖没有影响，而用脉冲低强度超声处理的样本中的胶原和糖胺聚糖含量始终更高，第 10 天有统计学显著差异（p < 0.05）。阿利新蓝染色显示，两组细胞周围均有糖胺聚糖沉积。这些结果表明，脉冲低强度超声对细胞增殖没有影响，但对培养在藻酸盐凝胶中的细胞诱导胶原和糖胺聚糖产生具有潜在作用。
