Wang Xiaoyan, Feng Mengli, Xiao Lu, Tong Aijun, Xiang Yu
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
ACS Chem Biol. 2016 Feb 19;11(2):444-51. doi: 10.1021/acschembio.5b00867. Epub 2015 Dec 16.
Photocaged (photoactivatable) biomolecules are powerful tools for noninvasive control of biochemical activities by light irradiation. DNAzymes (deoxyribozymes) are single-stranded oligonucleotides with a broad range of enzymatic activities. In this work, to construct photocaged DNAzymes, we developed a facile and mild postsynthetic method to incorporate an interesting photolabile modification (thioether-enol phosphate, phenol substituted, TEEP-OH) into readily available phosphorothioate DNA. Upon light irradiation, TEEP-OH transformed into a native DNA phosphodiester, and accordingly the DNAzymes with RNA-cleaving activities were turned "on" from its inactive and caged form. Activation of the TEEP-OH-caged DNAzyme by light was also successful inside live cells.
光笼（可光激活）生物分子是通过光照射对生化活性进行无创控制的强大工具。脱氧核酶是具有广泛酶活性的单链寡核苷酸。在这项工作中，为了构建光笼脱氧核酶，我们开发了一种简便温和的合成后方法，将一种有趣的光不稳定修饰（硫醚 - 烯醇磷酸酯，苯酚取代，TEEP - OH）引入易于获得的硫代磷酸酯DNA中。在光照射下，TEEP - OH转化为天然DNA磷酸二酯，相应地，具有RNA切割活性的脱氧核酶从其无活性的笼状形式转变为“开启”状态。TEEP - OH笼状脱氧核酶在活细胞内通过光激活也取得了成功。
