一种远红光荧光团的合成及其作为活细胞中酯酶探针的应用。
Synthesis of a far-red fluorophore and its use as an esterase probe in living cells.
作者信息
Levine Samantha R, Beatty Kimberly E
机构信息
Department of Biomedical Engineering, OHSU Center for Spatial Systems Biomedicine, Oregon Health & Science University, 2730 SW Moody Avenue, CL3B, Portland, OR 97201, USA.
出版信息
Chem Commun (Camb). 2016 Jan 31;52(9):1835-8. doi: 10.1039/c5cc08764c.
We report the synthesis of a new far-red fluorophore, 1,3-dichloro-7-hydroxy-2H-spiro[acridine-9,1'-cyclohexane]-2',5'-diene-2,4'-dione (DSACO), which was modified to make two esterase probes: DSACO-2-AME and DSACO-7-AME. Both probes act as "turn-on" substrates for esterases and lipases. DSACO-2-AME exhibited efficient esterase-activated fluorescence inside living cells and is a stable, far-red alternative for the widely-used fluorescein diacetate.
我们报道了一种新型远红光荧光团1,3-二氯-7-羟基-2H-螺[吖啶-9,1'-环己烷]-2',5'-二烯-2,4'-二酮（DSACO）的合成，该荧光团经修饰制成了两种酯酶探针：DSACO-2-AME和DSACO-7-AME。这两种探针均作为酯酶和脂肪酶的“开启型”底物。DSACO-2-AME在活细胞内表现出高效的酯酶激活荧光，是广泛使用的荧光素二乙酸酯的一种稳定的远红光替代物。
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