Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China.
Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention, College of Pharmacy, College of Integrative Medicine, Dalian Medical University, Dalian 116044, China.
Anal Chem. 2022 Aug 16;94(32):11216-11223. doi: 10.1021/acs.analchem.2c01691. Epub 2022 Aug 3.
Lipase found in the gut microbiota participates in the digestion and absorption of dietary fats. As such, the gut microbiota is involved in the regulation of the host metabolism, affecting the levels of lipids and free fatty acids, ultimately resulting in obesity. In this study, an enzymatic activatable near-infrared fluorescent probe, , was developed for visually sensing endogenous lipase from gut microbes. Using , a cultivated intestinal yeast strain was rapidly identified from human feces that exhibited high lipase expression and was identified as Y2. We then determined that the colonization of the gut of mice with Y2 increased lipase activity in the digestive tract and promoted obesity and hyperlipidemia when the mice were fed high fat diets. Above all, the present research resulted in a fluorescence visualization tool for the functional investigation of gut microbiota associated with obesity and disorders of lipid metabolism.
肠道微生物群中发现的脂肪酶参与膳食脂肪的消化和吸收。因此，肠道微生物群参与宿主代谢的调节，影响脂质和游离脂肪酸的水平，最终导致肥胖。在这项研究中，开发了一种酶激活的近红外荧光探针 ，用于可视化检测肠道微生物中的内源性脂肪酶。利用 ，从人类粪便中快速鉴定出一种具有高脂肪酶表达能力的培养肠道酵母菌株，并鉴定为 Y2。然后我们确定，用 Y2 定植小鼠肠道会增加肠道中的脂肪酶活性，并在给予高脂肪饮食时促进肥胖和高脂血症。总之，本研究为与肥胖和脂质代谢紊乱相关的肠道微生物群的功能研究提供了一种荧光可视化工具。
