Mifsud Antoinette, Peelen Daphne, Brincat Patricia, Abela Sylvana, Debattista Neville, Laspina Stefan, Zammit Daniel, Camilleri David J, Gatt Alex
Department of Haematology, Mater Dei Hospital, Msida, Malta.
Department of Haematology, Mater Dei Hospital, Msida, Malta VU University Amsterdam, Amsterdam, The Netherlands.
J Clin Pathol. 2016 Jul;69(7):637-42. doi: 10.1136/jclinpath-2015-203331. Epub 2015 Dec 15.
The aim of this study was to check the effect of Triton X-100 on various, commonly used haematology test parameters.
Anonymised blood samples were treated with 10 µL of 10% Triton X-100 per 1 mL of blood. Treated and untreated samples were tested in parallel for blood film morphology, complete blood counts (CBCs), flow cytometry, blood grouping and antibody screens. Samples were also taken in 3.2% citrate tubes for coagulation test analyses.
Statistical differences were noted in all CBC parameters apart from the mean cell volume, eosinophil and basophil counts. Platelet counts were significantly different with an apparent rise after the addition of Triton X-100. Samples were noted to have a high red cell fragmentation index. Immunological platelet counting methods using flow cytometry and fluorescent methods showed no significant differences and gave reliable results. Neither flow cytometry for T-cell subsets nor blood grouping/antibody screens were affected by Triton X-100. However, coagulation samples were severely haemolysed prohibiting analysis.
We have demonstrated that the addition of Triton X-100 to haematology blood samples impacts mainly on platelet counts and coagulation studies due to haemolysis. The platelet count is spuriously raised probably due to the presence of red cell fragments. The latter can be circumvented by the use of immunological platelet counting technology.
本研究旨在检测曲拉通X-100对各种常用血液学检测参数的影响。
将匿名血液样本每1 mL血液用10 μL 10%曲拉通X-100处理。对处理过的和未处理的样本同时进行血涂片形态学、全血细胞计数（CBC）、流式细胞术、血型鉴定和抗体筛查检测。样本还采集到3.2%枸橼酸盐试管中用于凝血试验分析。
除平均红细胞体积、嗜酸性粒细胞和嗜碱性粒细胞计数外，所有CBC参数均存在统计学差异。添加曲拉通X-100后血小板计数有明显升高，差异显著。样本的红细胞破碎指数较高。使用流式细胞术和荧光方法的免疫血小板计数方法无显著差异，结果可靠。曲拉通X-100对T细胞亚群的流式细胞术检测以及血型鉴定/抗体筛查均无影响。然而，凝血样本严重溶血，无法进行分析。
我们已经证明，向血液学血液样本中添加曲拉通X-100主要会因溶血而影响血小板计数和凝血研究。血小板计数可能因红细胞碎片的存在而假性升高。使用免疫血小板计数技术可以避免这种情况。
