Verma Anukriti, Singh Vinay Kumar, Gaur Smriti
Department of Biotechnology, Jaypee Institute of Information Technology (Deemed University), A-10, Sector-62, Noida, U.P., India.
Centre for Bioinformatics, School of Biotechnology, Banaras Hindu University, Varanasi, India.
Comput Biol Chem. 2016 Feb;60:53-8. doi: 10.1016/j.compbiolchem.2015.11.001. Epub 2015 Nov 10.
Phytase is an enzyme which catalyzes the total hydrolysis of phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate and digests the undigestable phytate part present in seeds and grains and therefore provides digestible phosphorus, calcium and other mineral nutrients. Phytases are frequently added to the feed of monogastric animals so that bioavailability of phytic acid-bound phosphate increases, ultimately enhancing the nutritional value of diets. The Bacillus phytase is very suitable to be used in animal feed because of its optimum pH with excellent thermal stability. Present study is aimed to perform an in silico comparative characterization and functional analysis of phytases from Bacillus amyloliquefaciens to explore physico-chemical properties using various bio-computational tools. All proteins are acidic and thermostable and can be used as suitable candidates in the feed industry.
植酸酶是一种能催化植酸盐完全水解为低磷酸化的肌醇衍生物和无机磷酸盐的酶，它能消化种子和谷物中难以消化的植酸盐部分，从而提供可消化的磷、钙和其他矿物质营养。植酸酶经常被添加到单胃动物的饲料中，以使植酸结合态磷酸盐的生物利用率提高，最终提升日粮的营养价值。芽孢杆菌植酸酶因其最适pH值和出色的热稳定性，非常适合用于动物饲料。本研究旨在对解淀粉芽孢杆菌的植酸酶进行计算机模拟比较表征和功能分析，利用各种生物计算工具探索其物理化学性质。所有蛋白质均呈酸性且具有热稳定性，可作为饲料行业的合适候选物。
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