Lian Jingjing, Ma Lili, Yang Jiayin, Xu Lili
Endoscopy Center, Zhongshan Hospital, Fudan University, Shanghai, China (mainland).
Division of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai, China (mainland).
Med Sci Monit. 2015 Dec 17;21:3935-40. doi: 10.12659/msm.895576.
BACKGROUND The aim of this study was to evaluate gene expression profiles in unaffected colon mucosa and polyp tissue from patients with unifocal colon polyp to investigate the potential mucosa impairment in normal-appearing colon mucosa from these patients. MATERIAL AND METHODS Colon polyp patients were prospectively recruited. We obtained colon biopsies from the normal-appearing sites and polyp tissue through colonoscopy. Gene expression analysis was performed using microarrays. Gene ontology and clustering were evaluated by bioinformatics. RESULTS We detected a total of 711 genes (274 up-regulated and 437 down-regulated) in polyp tissue and 256 genes (170 up-regulated and 86 down-regulated) in normal-appearing colon mucosa, with at least a 3-fold of change compared to healthy controls. Heatmapping of the gene expression showed similar gene alteration patterns between unaffected colon mucosa and polyp tissue. Gene ontology analyses confirmed the overlapped molecular functions and pathways of altered gene expression between unaffected colon mucosa and polyp tissue from patients with unifocal colon polyp. The most significantly altered genes in normal-appearing tissues in polyp patients include immune response, external side of plasma membrane, nucleus, and cellular response to zinc ion. CONCLUSIONS Significant gene expression alterations exist in unaffected colon mucosa from patients with unifocal colon polyp. Unaffected colon mucosa and polyp tissue share great similarity and overlapping of altered gene expression profiles, indicating the potential possibility of recurrence of colon polyps due to underlying molecular abnormalities of colon mucosa in these patients.
背景 本研究的目的是评估单发性结肠息肉患者未受影响的结肠黏膜和息肉组织中的基因表达谱，以调查这些患者外观正常的结肠黏膜中潜在的黏膜损伤情况。
材料与方法 前瞻性招募结肠息肉患者。通过结肠镜检查从外观正常的部位和息肉组织获取结肠活检样本。使用微阵列进行基因表达分析。通过生物信息学评估基因本体论和聚类情况。
结果 我们在息肉组织中总共检测到711个基因（274个上调和437个下调），在外观正常的结肠黏膜中检测到256个基因（170个上调和86个下调），与健康对照相比至少有3倍的变化。基因表达的热图显示未受影响的结肠黏膜和息肉组织之间存在相似的基因改变模式。基因本体论分析证实了单发性结肠息肉患者未受影响的结肠黏膜和息肉组织之间基因表达改变的重叠分子功能和途径。息肉患者外观正常组织中改变最显著的基因包括免疫反应、质膜外侧、细胞核以及对锌离子的细胞反应。
结论 单发性结肠息肉患者未受影响的结肠黏膜中存在显著的基因表达改变。未受影响的结肠黏膜和息肉组织在基因表达谱改变方面具有很大的相似性和重叠性，表明这些患者由于结肠黏膜潜在的分子异常存在结肠息肉复发的可能性。
