大鼠器官中腐胺生成γ-氨基丁酸的研究及其肠道合成酶的纯化

Studies on the formation of gamma-aminobutyric acid from putrescine in rat organs and purification of its synthetic enzyme from rat intestine.

作者信息

Tsuji M, Nakajima T

出版信息

J Biochem. 1978 May;83(5):1407-12. doi: 10.1093/oxfordjournals.jbchem.a132050.

DOI:10.1093/oxfordjournals.jbchem.a132050

Abstract

The formation of gamma-aminobutyric acid (GABA) from putrescine was examined in organs of rats using radioactive putrescine. Radioactive GABA was detected in all the organs of a rat injected intraperitoneally with radioactive putrescine, and the highest radioactivity of GABA was observed in the small intestine. The enzyme involved in this formation was purified from small intestine and identified as a diamine oxidase, histaminase, from the properties of the enzyme. Activity of the enzyme was found in all the organs of rat, and the highest activity was observed in the small intestine.

摘要

利用放射性腐胺研究了大鼠各器官中由腐胺形成γ-氨基丁酸（GABA）的过程。给大鼠腹腔注射放射性腐胺后，在其所有器官中均检测到放射性GABA，其中小肠中GABA的放射性最高。参与此形成过程的酶从小肠中纯化出来，并根据其酶的特性鉴定为二胺氧化酶，即组胺酶。在大鼠的所有器官中均发现了该酶的活性，其中小肠中的活性最高。