胰腺癌预后的综合评估：外周血中性粒细胞与淋巴细胞比值及肿瘤部位的免疫组化分析

Comprehensive assessment of the prognosis of pancreatic cancer: peripheral blood neutrophil-lymphocyte ratio and immunohistochemical analyses of the tumour site.

作者信息

Takakura Kazuki, Ito Zensho, Suka Machi, Kanai Tomoya, Matsumoto Yoshihiro, Odahara Shunichi, Matsudaira Hiroshi, Haruki Koichiro, Fujiwara Yuki, Saito Ryota, Gocho Takeshi, Nakashiro Koh-Ichi, Hamakawa Hiroyuki, Okamoto Masato, Kajihara Mikio, Misawa Takeyuki, Ohkusa Toshifumi, Koido Shigeo

机构信息

a Division of Gastroenterology and Hepatology, Department of Internal Medicine , Jikei University School of Medicine , Tokyo , Japan ;

b Department of Public Health and Environmental Medicine , Jikei University School of Medicine , Tokyo , Japan ;

出版信息

Scand J Gastroenterol. 2016;51(5):610-7. doi: 10.3109/00365521.2015.1121515. Epub 2015 Dec 17.

DOI:10.3109/00365521.2015.1121515

PMID:26679084

Abstract

OBJECTIVE

Several studies have suggested that an elevated neutrophil-lymphocyte ratio (NLR) is associated with a poorer prognosis in patients with pancreatic cancer (PC). The correlations between the NLR and immunohistochemical (IHC) analysis with regard to the prognosis of patients with PC remain to be elucidated. By using IHC findings, we determined the value of the NLR as a prognostic factor in patients with PC.

MATERIAL AND METHODS

We collected the clinico-pathological data of 28 consecutive patients who underwent surgical resection for PC between January 2008 and December 2012 at The Jikei University Kashiwa Hospital. We investigated whether the NLR and IHC results were related and ensured the consistency of the prognosis of patients with PC.

RESULTS

The Kaplan-Meier curves for the disease-free survival (DFS) and the overall survival (OS) revealed that an NLR ≥ 5 is an implicit factor for decreased DFS and OS in patients with PC (p = 0.003, p < 0.001, log-rank test). The density of CD163(+) macrophages and CD66b(+) neutrophils was significantly higher in the high NLR group; on the contrary, the density of CD20(+) lymphocytes was significantly higher in the low NLR group. Moreover, a Mann-Whitney U test showed that the NLR was significantly correlated with a high density of CD20(+) lymphocytes (p = 0.031) and CD163(+) macrophages (p = 0.023), while the NLR was not significantly correlated with CD66b(+) neutrophils (p = 0.397).

CONCLUSIONS

Our results demonstrated the validity of the NLR by IHC analyses and we determined that a higher value of NLR is a trustworthy prognostic factor for patients with PC.

摘要

目的

多项研究表明，中性粒细胞与淋巴细胞比值（NLR）升高与胰腺癌（PC）患者预后较差相关。NLR与免疫组织化学（IHC）分析在PC患者预后方面的相关性仍有待阐明。通过使用IHC结果，我们确定了NLR作为PC患者预后因素的价值。

材料与方法

我们收集了2008年1月至2012年12月期间在北里大学柏医院接受PC手术切除的28例连续患者的临床病理数据。我们研究了NLR和IHC结果是否相关，并确保了PC患者预后的一致性。

结果

无病生存期（DFS）和总生存期（OS）的Kaplan-Meier曲线显示，NLR≥5是PC患者DFS和OS降低的一个隐含因素（p = 0.003，p < 0.001，对数秩检验）。高NLR组中CD163(+)巨噬细胞和CD66b(+)中性粒细胞的密度显著更高；相反，低NLR组中CD20(+)淋巴细胞的密度显著更高。此外，Mann-Whitney U检验显示，NLR与CD20(+)淋巴细胞的高密度（p = 0.031）和CD163(+)巨噬细胞的高密度（p = 0.023）显著相关，而NLR与CD66b(+)中性粒细胞无显著相关性（p = 0.397）。