Salomone Alberto, Gazzilli Giulia, Di Corcia Daniele, Gerace Enrico, Vincenti Marco
Centro Regionale Antidoping e di Tossicologia "A. Bertinaria", Regione Gonzole 10/1, 10043, Orbassano, TO, Italy.
Dipartimento di Chimica, Università degli Studi di Torino, via P. Giuria 7, 10125, Turin, Italy.
Anal Bioanal Chem. 2016 Mar;408(8):2035-42. doi: 10.1007/s00216-015-9247-4. Epub 2015 Dec 17.
The detection of new psychoactive substances (NPS) in hair proved to provide insight into their current diffusion among the population and the social characteristics of these synthetic drugs' users. Therefore, a UHPLC-MS/MS method was developed in order to determine 31 stimulant and psychedelic substituted phenethylamines, and dissociative drugs in hair samples. The method proved to be simple, fast, specific, and sensitive. The absence of matrix interferents, together with excellent repeatability of both retention times and relative abundances of diagnostic transitions, allowed the correct identification of all analytes tested. The method showed optimal linearity in the interval 10-1000 pg/mg, with correlation coefficient values varying between 0.9981 and 0.9997. Quantitation limits ranged from 1.8 pg/mg for 4-methoxyphencyclidine (4-MeO-PCP) up to 35 pg/mg for 6-(2-aminopropyl)benzofuran (6-APB). The method was applied to (i) 23 real samples taken from proven MDMA and ketamine abusers and (ii) 54 real hair samples which had been previously tested negative during regular drug screening in driver's license recovery. Six samples tested positive for at least one target analyte. Methoxetamine (MXE) was found in three cases (range of concentration 7.7-27 pg/mg); mephedrone (4-MMC) was found in two cases (50-59 pg/mg) while one sample tested positive for methylone at 28 pg/mg. Other positive findings included 4-methylethcathinone (4-MEC), alpha-pyrrolidinovalerophenone (α-PVP), 4-fluoroamphetamine (4-FA), 3,4-methylenedioxypyrovalerone (MDPV), and diphenidine. The present study confirms the increasing diffusion of new designer drugs with enhanced stimulant activity among the target population of poly-abuse consumers.
毛发中新精神活性物质（NPS）的检测被证明有助于深入了解它们目前在人群中的传播情况以及这些合成药物使用者的社会特征。因此，开发了一种超高效液相色谱-串联质谱（UHPLC-MS/MS）方法，用于测定毛发样本中的31种刺激性和致幻性取代苯乙胺及分离性药物。该方法被证明简单、快速、特异且灵敏。由于不存在基质干扰物，同时保留时间和诊断离子相对丰度具有出色的重复性，所有测试分析物均能得到正确鉴定。该方法在10 - 1000 pg/mg区间内呈现出最佳线性，相关系数值在0.9981至0.9997之间。定量限范围从4-甲氧基苯环利定（4-MeO-PCP）的1.8 pg/mg到6-(2-氨基丙基)苯并呋喃（6-APB）的35 pg/mg。该方法应用于：（i）从已证实滥用摇头丸和氯胺酮的使用者中采集的23份真实样本，以及（ii）54份在驾照恢复常规药物筛查中先前检测为阴性的真实毛发样本。六个样本至少对一种目标分析物呈阳性。在三个案例中发现了甲氧麻黄酮（MXE）（浓度范围为7.7 - 27 pg/mg）；在两个案例中发现了甲麻黄碱（4-MMC）（50 - 59 pg/mg），而一个样本中甲基酮呈阳性，浓度为28 pg/mg。其他阳性结果包括4-甲基乙卡西酮（4-MEC）、α-吡咯烷戊酮（α-PVP）、4-氟苯丙胺（4-FA）、3,4-亚甲基二氧吡咯戊酮（MDPV）和二苯哌啶。本研究证实，具有增强刺激活性的新型设计药物在多药滥用消费者目标人群中的传播日益增加。
