CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Carbohydr Polym. 2016 Feb 10;137:382-389. doi: 10.1016/j.carbpol.2015.10.075. Epub 2015 Oct 26.
Chitosan (Chit) was submitted to the Maillard reaction (MR) by co-heating a solution with glucose (Glc). Different reaction conditions as temperature (40, 60 and 80 °C), Glc concentration (0.5%, 1%, and 2%, w/v), and reaction time (72, 52 and 24h) were evaluated. Assessment of the reaction extent was monitored by measuring changes in UV absorbance, browning and fluorescence. Under the best conditions, 2% (w/v) of Chit, 2% (w/v) of Glc at 60°C and 32 h of reaction time, a chitosan-glucose (Chit-Glc) derivative was purified and submitted to structural characterization to confirm its formation. Analysis of its molecular weight (MW) and the degree of substitution (DS) was carried out by HPLC-Size Exclusion Chromatography (SEC) and a colloid titration method, respectively. FT-IR and (1)H NMR were also used to analyze the functional groups and evaluate the introduction of Glc into the Chit molecule. According to our objectives, the results obtained in this work allowed to better understand the key parameters influencing the MR with Chit as well as to confirm the successful introduction of Glc into the Chit molecule obtaining a Chit-Glc derivative with a DS of 64.76 ± 4.40% and a MW of 210.37 kDa.
壳聚糖（Chit）与葡萄糖（Glc）共热，进行美拉德反应（MR）。评估了不同的反应条件，如温度（40、60 和 80°C）、Glc 浓度（0.5%、1%和 2%，w/v）和反应时间（72、52 和 24h）。通过测量 UV 吸光度、褐变和荧光的变化来监测反应程度。在最佳条件下，即 2%（w/v）的 Chit、2%（w/v）的 Glc 在 60°C 下反应 32 小时，纯化壳聚糖-葡萄糖（Chit-Glc）衍生物，并对其结构进行了表征，以确认其形成。通过高效液相色谱-尺寸排阻色谱（SEC）和胶体滴定法分别分析其分子量（MW）和取代度（DS）。还使用傅里叶变换红外光谱（FT-IR）和（1）H NMR 分析了功能基团，并评估了 Glc 引入 Chit 分子中的情况。根据我们的目标，这项工作的结果使我们能够更好地了解影响 Chit 美拉德反应的关键参数，并确认成功地将 Glc 引入 Chit 分子中，得到了取代度为 64.76±4.40%、分子量为 210.37 kDa 的 Chit-Glc 衍生物。
