College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Department of Orthopaedic Surgery, Chiayi Chang Gung Memorial Hospital, No. 6, West Sec., Chia-Pu Rd., Pu-Tz City, Chiayi County, 61363, Taiwan.
J Orthop Surg Res. 2024 Oct 5;19(1):628. doi: 10.1186/s13018-024-05127-7.
Treatment of articular cartilage injury remains a challenging clinical problem in orthopedics. Chitosan-derived biomaterial could be a potential adjuvant treatment to improve cartilage repair. In the current study, we examined the effects of two potential chitosan-derived materials on cartilage regeneration of osteochondral defects in rabbits.
An osteochondral defect was created over the rabbit knee and treated using three approaches: group A received no material (n = 24), group B received chitosan membranes with glucose absorption (CGA; n = 25), and group C received chitosan-glucose derivative membranes obtained via the Maillard reaction (CGMR; n = 25). Cartilage repair over the osteochondral defect was analyzed 12 weeks post-surgery via histological analysis, immunostaining, and reverse transcription-qualitative polymerase chain reaction (RT-qPCR) for type-I and type-II collagen mRNA.
According to histological analysis, CGMR-treated defects showed significantly improved modified O'Driscoll scoring when compared with no material- and CGA-treated defects (20.9 ± 4.3 vs. 13.00 ± 2.5 and 17.7 ± 4.6, p < 0.001). Moreover, group C exhibited higher intensity of type-II collagen immunohistochemical staining over the regenerated cartilage than groups A and B, along with increased expression of type-II collagen mRNA by RT-qPCR.
CGMR might improve cartilage regeneration in osteochondral defects.
关节软骨损伤的治疗仍然是骨科领域的一个具有挑战性的临床问题。壳聚糖衍生的生物材料可能是一种潜在的辅助治疗方法，以改善软骨修复。在本研究中，我们研究了两种潜在的壳聚糖衍生材料对兔骨软骨缺损软骨再生的影响。
在兔膝关节上创建一个骨软骨缺损，并采用三种方法进行治疗：A 组不接受任何材料（n=24），B 组接受具有葡萄糖吸收能力的壳聚糖膜（CGA；n=25），C 组接受通过美拉德反应获得的壳聚糖-葡萄糖衍生物膜（CGMR；n=25）。术后 12 周通过组织学分析、免疫染色和 I 型和 II 型胶原 mRNA 的逆转录定量聚合酶链反应（RT-qPCR）分析骨软骨缺损处的软骨修复情况。
根据组织学分析，与无材料和 CGA 处理的缺陷相比，CGMR 处理的缺陷的改良 O'Driscoll 评分显著提高（20.9±4.3 比 13.00±2.5 和 17.7±4.6，p<0.001）。此外，与 A 组和 B 组相比，C 组在再生软骨中 II 型胶原免疫组化染色强度更高，同时 RT-qPCR 检测到 II 型胶原 mRNA 的表达增加。
CGMR 可能改善骨软骨缺损中的软骨再生。
