印度部落的肠道细菌多样性及其与全球数据的比较。
Gut bacterial diversity of the tribes of India and comparison with the worldwide data.
作者信息
Dehingia Madhusmita, Devi Kanchal Thangjam, Talukdar Narayan C, Talukdar Rupjyoti, Reddy Nageshwar, Mande Sharmila S, Deka Manab, Khan Mojibur R
机构信息
Molecular Biology and Microbial Biotechnology Laboratory, Life Science Division, Institute of Advanced Study in Science and Technology (IASST), An autonomous institute under Department of Science and Technology (Govt. of India), Paschim Boragaon, Garchuk, Guwahati-781035, Assam, India.
Institute of Bioresources &Sustainable Development (IBSD), An autonomous institute under Department of Biotechnology (Govt. of India), Takyelpat, Imphal-795001, Manipur, India.
出版信息
Sci Rep. 2015 Dec 22;5:18563. doi: 10.1038/srep18563.
The gut bacteria exert phenotypic traits to the host but the factors which determine the gut bacterial profile (GBP) is poorly understood. This study aimed to understand the effect of ethnicity and geography on GBP of Mongoloid and Proto-Australoid tribes of India. Fecal bacterial diversity was studied in fifteen tribal populations representing four geographic regions (Assam, Telangana, Manipur and Sikkim) by DGGE followed by NGS analysis on Illumina MiSeq platform. Geography and diet had significant effect on GBP of the Indian tribes which was dominated by Prevotella. The effects were more prominent with lower taxonomic levels, indicating probable functional redundancy of the core GBP. A comparison with the worldwide data revealed that GBP of the Indian population was similar to the Mongolian population (Mongolia). The bacterial genera Faecalibacterium, Eubacterium, Clostridium, Blautia, Ruminococcus and Roseburia were found to be core genera in the representative populations of the world.
肠道细菌会对宿主表现出表型特征，但决定肠道细菌谱（GBP）的因素却知之甚少。本研究旨在了解种族和地理位置对印度蒙古人种和澳大利亚原住民部落GBP的影响。通过变性梯度凝胶电泳（DGGE），随后在Illumina MiSeq平台上进行二代测序（NGS）分析，研究了代表四个地理区域（阿萨姆邦、特伦甘纳邦、曼尼普尔邦和锡金邦）的15个部落人群的粪便细菌多样性。地理位置和饮食对以普雷沃氏菌为主的印度部落的GBP有显著影响。在较低的分类水平上，这种影响更为显著，表明核心GBP可能存在功能冗余。与全球数据的比较显示，印度人群的GBP与蒙古人群（蒙古国）相似。在世界代表性人群中，发现粪杆菌属、真杆菌属、梭菌属、布劳特氏菌属、瘤胃球菌属和罗斯氏菌属为核心菌属。
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