Song Can, Wang Bochu, Tan Jun, Zhu Liancai, Lou Deshuai, Cen Xiaoxi
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.
Bioengineering College, Chongqing University, No. 174, Shapingba Main Street, Chongqing, 400030, China.
Mol Genet Genomics. 2017 Apr;292(2):407-414. doi: 10.1007/s00438-016-1282-0. Epub 2016 Dec 27.
The Asiatic black bear (Ursus thibetanus) is a protected species from eastern Asia. In China, the Asiatic black bear occurs in 17 provinces from northeast to southwest regions. To date, information on microbial diversity in the gut of the Asiatic black bears from different populations remains limited. To determine the species composition and community structure of the gut microbiota in the Asiatic black bear, we characterized 36 fecal samples from Sichuan, Yunnan, and Heilongjiang provinces, China, by pyrosequencing the 16S V3-V4 hypervariable regions using the Illumina Miseq platform. Results showed that Firmicutes and Proteobacteria were the predominant phyla in the samples, which were largely comprised Escherichia-Shigella, Peptostreptococcaceae_incertae_sedis, Turicibacter, Streptococcus, and Clostridium. By analyzing the community structure from these 36 samples, we found that there were significant differences in the species diversity and richness between Sichuan, Yunnan, and Heilongjiang populations. In conclusion, our results reveal the species composition and structure of the gut microbiota in captive black bears in China, and suggest that biogeography could affect the black bear' gut microbiota.
亚洲黑熊（Ursus thibetanus）是一种来自东亚的受保护物种。在中国，亚洲黑熊分布于从东北到西南地区的17个省份。迄今为止，关于不同种群亚洲黑熊肠道微生物多样性的信息仍然有限。为了确定亚洲黑熊肠道微生物群的物种组成和群落结构，我们利用Illumina Miseq平台对中国四川、云南和黑龙江三省的36份粪便样本进行16S V3-V4高变区焦磷酸测序，从而对其进行了特征分析。结果显示，厚壁菌门和变形菌门是样本中的主要门类，主要包括大肠杆菌-志贺氏菌属、消化链球菌科未定种、Turicibacter、链球菌属和梭菌属。通过分析这36个样本的群落结构，我们发现四川、云南和黑龙江种群之间的物种多样性和丰富度存在显著差异。总之，我们的结果揭示了中国圈养黑熊肠道微生物群的物种组成和结构，并表明生物地理学可能会影响黑熊的肠道微生物群。
