Tanaskovic Marija, Novicic Zorana Kurbalija, Kenig Bojan, Stamenkovic-Radak Marina, Andjelkovic Marko
Faculty of Biology, University of Belgrade, Studentski Trg 3, 11000 Belgrade, Republic of Serbia.
J Genet. 2015 Dec;94(4):643-9. doi: 10.1007/s12041-015-0569-y.
Lead is one of the most present contaminants in the environment, and different species respond differently to this type of polution. If combined with genomic stress, lead may act synergistically, causing significant decrease of fitness components. We used two genetically diverse Drosophila subobscura populations (regarding both putatively adaptive inversion and microsatellite loci polymorphisms) originating from two ecologically distinct habitats. To establish different levels of genome heterozygosity, series of intraline, intrapopulation and interpopulation crosses were made. The progeny were reared on a standard medium and a medium with 200 μg/mL of lead acetate. Development time was significantly extended to all groups reared on lead. The progeny of intraline crosses showed significantly extended development time compared to all other groups. The obtained results suggest that genome heterozygosity reduces the effect of lead pollution.
铅是环境中最常见的污染物之一，不同物种对这种污染的反应各不相同。如果与基因组应激相结合，铅可能会产生协同作用，导致适应性成分显著下降。我们使用了来自两个生态截然不同栖息地的两个遗传多样的果蝇亚暗果蝇种群（关于假定的适应性倒位和微卫星位点多态性）。为了建立不同水平的基因组杂合性，进行了一系列品系内、种群内和种群间杂交。后代在标准培养基和含有200μg/mL醋酸铅的培养基上饲养。发育时间在所有用铅饲养的组中显著延长。与所有其他组相比，品系内杂交的后代发育时间显著延长。所得结果表明，基因组杂合性降低了铅污染的影响。
