Beribaka Mirjana, Jelić Mihailo, Tanasković Marija, Lazić Cvijeta, Stamenković-Radak Marina
Faculty of Technology Zvornik, University of East Sarajevo, Karakaj 34A, 75400 Zvornik, Bosnia and Herzegovina.
Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
Insects. 2021 Dec 15;12(12):1122. doi: 10.3390/insects12121122.
Life history traits determine the persistence and reproduction of each species. Factors that can affect life history traits are numerous and can be of different origin. We investigated the influence of population origin and heavy metal exposure on microbiota diversity and two life history traits, egg-to-adult viability and developmental time, in and grown in the laboratory on a lead (II) acetate-saturated substrate. We used 24 samples, 8 larval and 16 adult samples (two species × two substrates × two populations × two sexes). The composition of microbiota was determined by sequencing (NGS) of the V3-V4 variable regions of the 16S rRNA gene. The population origin showed a significant influence on life history traits, though each trait in the two species was affected differentially. Reduced viability in could be a cost of fast development, decrease in abundance and the presence of . The heavy metal exposure in caused shifts in developmental time but maintained the egg-to-adult viability at a similar level. Microbiota diversity indicated that the could be a valuable member of microbiota in overcoming the environmental stress. Research on the impact of microbiota on the adaptive response to heavy metals and consequently the potential tradeoffs among different life history traits is of great importance in evolutionary research.
生活史特征决定了每个物种的持久性和繁殖能力。能够影响生活史特征的因素众多，且来源各异。我们研究了种群来源和重金属暴露对在实验室中于醋酸铅（II）饱和底物上生长的[物种名称1]和[物种名称2]的微生物群多样性以及两个生活史特征（卵到成虫的存活率和发育时间）的影响。我们使用了24个样本，包括8个幼虫样本和16个成虫样本（两个物种×两种底物×两个种群×两种性别）。通过对16S rRNA基因的V3 - V4可变区进行测序（NGS）来确定微生物群的组成。种群来源对生活史特征有显著影响，尽管两个物种的每个特征受到的影响有所不同。[物种名称1]中存活率降低可能是快速发育的代价、[微生物名称1]丰度的降低以及[微生物名称2]的存在所致。[物种名称2]中的重金属暴露导致发育时间发生变化，但卵到成虫的存活率维持在相似水平。微生物群多样性表明，[微生物名称3]可能是[物种名称2]微生物群中克服环境压力的重要成员。研究微生物群对重金属适应性反应的影响以及因此在不同生活史特征之间潜在的权衡，在进化研究中具有重要意义。
