Vardavas Alexander I, Fragkiadaki Persefoni, Alegakis Athanasios K, Kouretas Dimitrios, Goutzourelas Nikolaos, Tsiaoussis John, Tsitsimpikou Christina, Stivaktakis Polychronis D, Carvalho Félix, Tsatsakis Aristidis M
Laboratory of Toxicology, Medical School, University of Crete, Voutes, 71409 Heraklion, Crete, Greece.
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou St., Larissa 41221, Greece.
Life Sci. 2016 Jan 15;145:114-20. doi: 10.1016/j.lfs.2015.12.026. Epub 2015 Dec 12.
The aimof this study is to clarify the effect of cypermethrin (CY) on the oxidative stress (OS) and inflammation status of animals exposed to it and the synergistic role of piperonyl butoxide (PB0).
Markers of oxidative stress, such as total antioxidant activity (TAC), protein carbonyls, hemoglobin (Hb), reduced glutathione (GSH), thiobarbituric-acid reactive substances (TBARS), along with the telomerase activity in PBMCs (peripheral blood mononuclear cells) were analyzed.
Oxidative stress markers showed statistically significant differences between groups in TAC (p b 0.001), GSH (p = 0.018) and CAT activity (p = 0.029), which depended on dose and combined effect of both compounds. Telomerase activity also showed a statistically significant difference between all groups (F = 43.48, df=6, 14, p b 0.001)with cypermethrin, piperonyl butoxide and the co-exposed groups being significantly different fromthe control group (p b 0.05). Significance: The observed results for TBARS, GSH, Hb, TAC, Crbnls and CAT from our exposed groups showed altered levels compared to control groups that could be linked to doses and combined effects of each chemical substance (cypermethrin and piperonyl butoxide). Oxidative stress markers suggest that cypermethrin, piperonyl butoxide and the co-exposed groups, induce oxidative stress as well as induction of telomerase activity.
本研究旨在阐明氯氰菊酯（CY）对接触该物质的动物氧化应激（OS）和炎症状态的影响以及胡椒基丁醚（PB0）的协同作用。
分析了氧化应激标志物，如总抗氧化活性（TAC）、蛋白质羰基、血红蛋白（Hb）、还原型谷胱甘肽（GSH）、硫代巴比妥酸反应性物质（TBARS），以及外周血单核细胞（PBMCs）中的端粒酶活性。
氧化应激标志物在TAC（p<0.001）、GSH（p = 0.018）和CAT活性（p = 0.029）方面显示出组间具有统计学意义的差异，这取决于两种化合物的剂量和联合效应。端粒酶活性在所有组间也显示出统计学意义的差异（F = 43.48，df = 6, 14，p<0.001），氯氰菊酯、胡椒基丁醚及联合暴露组与对照组有显著差异（p<0.05）。意义：与对照组相比，我们的暴露组中观察到的TBARS、GSH、Hb、TAC、Crbnls和CAT结果显示水平发生了变化，这可能与每种化学物质（氯氰菊酯和胡椒基丁醚）的剂量和联合效应有关。氧化应激标志物表明，氯氰菊酯、胡椒基丁醚及联合暴露组会诱导氧化应激以及端粒酶活性。
