Cao Binrui, Yang Mingying, Wang Lin, Xu Hong, Zhu Ye, Mao Chuanbin
Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019-5251 USA.
Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang 310058, China.
J Mater Chem B. 2015 Oct 21;3(39):7667-7672. doi: 10.1039/C5TB01509J. Epub 2015 Sep 3.
Synthetic nanoparticles are always terminated with coating molecules, which are often cytotoxic and not desired in biomedicine. Here we propose a novel reaction-dissolution approach to remove the cytotoxic coating molecules. A two-component solution is added to the nanoparticle solution; one component reacts with the coating molecules to form a salt whereas another is a solvent for dissolving and thus removing the salt. As a proof of concept, this work uses a NaOH-ethanol solution to remove the cytotoxic linoleic acid molecules coated on the hydroxyapatite nanorods (HAP-NRs). The removal of the coating molecules not only significantly improves the biocompatibility of HAP-NRs but also enables their oriented attachment into tightly-bound superstructures, which mimic the organized HAP crystals in bone and enamel and can promote the osteogenic differentiation of mesenchymal stem cells. Our reaction-dissolution approach can be extended to the surface "cleaning" of other nanomaterials.
合成纳米颗粒总是被包覆分子所终止，这些包覆分子通常具有细胞毒性，在生物医学中是不需要的。在此，我们提出一种新颖的反应溶解方法来去除细胞毒性包覆分子。将一种双组分溶液添加到纳米颗粒溶液中；一种组分与包覆分子反应形成盐，而另一种是用于溶解并因此去除该盐的溶剂。作为概念验证，这项工作使用氢氧化钠 - 乙醇溶液去除包覆在羟基磷灰石纳米棒（HAP - NRs）上的细胞毒性亚油酸分子。包覆分子的去除不仅显著提高了HAP - NRs的生物相容性，还使其能够定向附着形成紧密结合的超结构，这种超结构模仿了骨骼和牙釉质中有组织的HAP晶体，并且可以促进间充质干细胞的成骨分化。我们的反应溶解方法可以扩展到其他纳米材料的表面“清洁”。
