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miR-125a和miR-125b的高甲基化相关沉默：结直肠癌的一种潜在标志物

Hypermethylation-Associated Silencing of miR-125a and miR-125b: A Potential Marker in Colorectal Cancer.

作者信息

Chen Hui, Xu Zhiying

机构信息

Department of Gastroenterology, People's Hospital of Taizhou, 399 Hailing Road, Taizhou, Jiangsu 225300, China.

Department of Gastroenterology, People's Hospital of Taizhou, 399 Hailing Road, Taizhou, Jiangsu 225300, China ; Department of Gastroenterology, Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha, Hunan 410011, China.

出版信息

Dis Markers. 2015;2015:345080. doi: 10.1155/2015/345080. Epub 2015 Nov 26.

DOI:10.1155/2015/345080
PMID:26693202
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4674596/
Abstract

BACKGROUND

MicroRNAs (miRNAs) have been found to be downregulated in human colorectal cancer (CRC), and some of them may function as tumor suppressor genes (TSGs). Aberrant methylation triggers the inactivation of TSGs during tumorigenesis.

PATIENTS AND METHODS

We investigated the methylation status of miR-125 family in CRC tissues and adjacent nontumor tissues by using bisulfite sequencing PCR (BSP). The expression levels of the two miRNAs were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results. The methylation frequency of miR-125a and miR-125b was higher in CRC tissues. QRT-PCR analysis showed that miR-125a and miR-125b were significantly downregulated in CRC tissues. Moreover, the expression levels of miR-125a and miR-125b were inversely correlated to CpG island methylation in CRC.

CONCLUSIONS

Our results suggest that DNA hypermethylation may be involved in the inactivation of miR-125a and miR-125b in CRC, and hypermethylation of miR-125 is a potential biomarker for clinical outcome.

摘要

背景

微小RNA（miRNA）在人类结直肠癌（CRC）中被发现表达下调，其中一些可能作为肿瘤抑制基因（TSG）发挥作用。异常甲基化在肿瘤发生过程中触发TSG的失活。

患者和方法

我们通过亚硫酸氢盐测序PCR（BSP）研究了结直肠癌组织和相邻非肿瘤组织中miR-125家族的甲基化状态。通过定量逆转录聚合酶链反应（qRT-PCR）测定这两种miRNA的表达水平。结果。miR-125a和miR-125b在结直肠癌组织中的甲基化频率更高。qRT-PCR分析表明，miR-125a和miR-125b在结直肠癌组织中显著下调。此外，结直肠癌中miR-125a和miR-125b的表达水平与CpG岛甲基化呈负相关。

结论

我们的结果表明，DNA高甲基化可能参与了结直肠癌中miR-125a和miR-125b的失活，miR-125的高甲基化是临床结局的潜在生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/f155d6315446/DM2015-345080.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/adf4a19f626b/DM2015-345080.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/e73345fa5fa0/DM2015-345080.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/f4fcb1352462/DM2015-345080.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/f155d6315446/DM2015-345080.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/adf4a19f626b/DM2015-345080.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/e73345fa5fa0/DM2015-345080.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/f4fcb1352462/DM2015-345080.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df23/4674596/f155d6315446/DM2015-345080.004.jpg

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