环状RNA CASC15-微小RNA-100-雷帕霉素靶蛋白复合物可能影响宫颈癌的放射抗性。
CircCASC15-miR-100-mTOR may influence the cervical cancer radioresistance.
作者信息
Yao Tingting, Yao Yao, Chen Zhiliao, Peng Yongpai, Zhong Guanglei, Huang Chunxian, Li Jing, Li Ruixin
机构信息
Department of Gynecological Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People's Republic of China.
Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun Yat-Sen University, Guangzhou, China.
出版信息
Cancer Cell Int. 2022 Apr 27;22(1):165. doi: 10.1186/s12935-022-02573-3.
BACKGROUND
Cervical cancer has ranked the top one in gynecological malignancies for incidence. Radioresistance is now becoming a leading reason of recurrence.
METHODS
Our microRNA array data indicated that the miRNA-100 level decreased significantly during radioresistance. In this study, we up-regulated miR-100 in Hela and Siha cells by using miR-100 mimics and observed proliferation and invasion.
RESULTS
It turned out that with overexpression of miR-100, the cells had less invasiveness as well as proliferation. It may target gene mTOR, and it deed reduced EMT. To examine the role of miR-100 in radioresistance, there was no significant result showed by BSP. While the circCASC15 has been identified with sponge function according to RNA pull down and ISH.
CONCLUSION
The conclusions indicate miR-100 is a tumor suppressor gene and could be a therapeutic target in radio-resistant cervical cancers.
背景
宫颈癌的发病率在妇科恶性肿瘤中位居首位。放射抗性正成为复发的主要原因。
方法
我们的 microRNA 芯片数据表明，在放射抗性过程中 miRNA-100 水平显著降低。在本研究中，我们通过使用 miR-100 模拟物上调 Hela 和 Siha 细胞中的 miR-100，并观察其增殖和侵袭情况。
结果
结果表明，随着 miR-100 的过表达，细胞的侵袭性和增殖能力降低。它可能靶向基因 mTOR，并且确实减少了上皮间质转化。为了研究 miR-100 在放射抗性中的作用，BSP 未显示出显著结果。而根据 RNA 下拉和原位杂交，circCASC15 已被确定具有海绵功能。
结论
结论表明 miR-100 是一种肿瘤抑制基因，可能成为放射抗性宫颈癌的治疗靶点。
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