Giessler C, Panse M, Mentz P, Hellthaler G
Z Med Lab Diagn. 1989;30(3):175-9.
A sensitive and specific enzyme immunoassay for TXB2, the stable degradation product of thromboxane A2, was developed, in which the hapten molecule was labeled with pure peroxidase. After competitive binding to antibody between enzyme-labeled and free TXB2, the immunoreactive product was precipitated by double antibody technique, and the enzyme activity of the precipitate was determined spectrophotometrically. The procedures allowed a determination of 3-200 pg TXB2/tube (0.081 to 5.4 nmol/l).
开发了一种用于检测血栓素A2的稳定降解产物TXB2的灵敏且特异的酶免疫测定法，其中半抗原分子用纯过氧化物酶标记。酶标记的TXB2和游离TXB2与抗体竞争性结合后，采用双抗体技术沉淀免疫反应产物，并用分光光度法测定沉淀物的酶活性。该方法可测定3 - 200 pg TXB2/管（0.081至5.4 nmol/L）。
