Hinman Samuel S, Chen Chih-Yuan, Duan Jicheng, Cheng Quan
Environmental Toxicology, University of California, Riverside, California 92521, USA.
Department of Chemistry, University of California, Riverside, California 92521, USA.
Nanoscale. 2016 Jan 21;8(3):1665-75. doi: 10.1039/c5nr06635b.
A patterned gold nanoparticle microarray, functionalized with a nanoscale silicate coating, has been developed for on-chip, high-throughput mass spectrometric analyses of biomolecules with minimal sample preparation and reagent costs. Fabrication was realized by the combination of layer-by-layer functionalization of the nanoparticles with suitable polyelectrolytes, followed by fluidic patterning of the glass microarray support and calcination for permanent fixation of the nano-coating. Performance of the microarray was evaluated for surface-assisted laser-desorption/ionization mass spectrometry (SALDI-MS), where the nano-silicate coating was found to enhance SALDI efficiency, resulting in comparable performance to some common organic matrices for small and medium sized molecules. Performance contributing factors of this material have been discussed; heat confinement and interband transition/plasmonic resonance may play important roles. Taking the accessibility of fabrication, performance, and reusability of this substrate together, the material developed here provides a new tool for multiplexed and chip-based mass spectrometric analysis.
一种用纳米级硅酸盐涂层功能化的图案化金纳米颗粒微阵列已被开发出来，用于对生物分子进行片上高通量质谱分析，同时将样品制备和试剂成本降至最低。通过将纳米颗粒与合适的聚电解质进行逐层功能化，然后对玻璃微阵列载体进行流体图案化，并进行煅烧以永久固定纳米涂层，实现了微阵列的制备。对该微阵列进行了表面辅助激光解吸/电离质谱（SALDI-MS）性能评估，发现纳米硅酸盐涂层可提高SALDI效率，对于中小分子而言，其性能与一些常见有机基质相当。讨论了这种材料的性能影响因素；热限制和带间跃迁/等离子体共振可能起重要作用。综合考虑该基质的制备可及性、性能和可重复使用性，本文开发的材料为基于芯片的多重质谱分析提供了一种新工具。
