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一种来自芽孢杆菌属的抗菌代谢产物：对包括多重耐药临床菌株在内的致病细菌具有显著活性。

An Antimicrobial Metabolite from Bacillus sp.: Significant Activity Against Pathogenic Bacteria Including Multidrug-Resistant Clinical Strains.

作者信息

Chalasani Ajay G, Dhanarajan Gunaseelan, Nema Sushma, Sen Ramkrishna, Roy Utpal

机构信息

Department of Biological Sciences, BITS Pilani- K. K. Birla Goa Campus Goa, India.

Department of Biotechnology, Indian Institute of Technology-Kharagpur Kharagpur, India.

出版信息

Front Microbiol. 2015 Dec 15;6:1335. doi: 10.3389/fmicb.2015.01335. eCollection 2015.

DOI:10.3389/fmicb.2015.01335
PMID:26696963
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4678185/
Abstract

In this study, the cell free modified tryptone soya broth (pH 7.4 ± 0.2) of Bacillus subtilis URID 12.1 showed significant antimicrobial activity against multidrug-resistant strains of Staphylococcus aureus, S. epidermidis, Streptococcus pyogenes and Enterococcus faecalis. The partially purified antimicrobial molecule was found to be resistant to extremes of pH and temperatures and also to higher concentrations of trypsin and proteinase K. The antimicrobial molecule was purified by a three-step method that included reversed-phase high performance liquid chromatography (RP-HPLC). The minimum inhibitory concentration (MIC) values were determined for 14 species of bacteria using a microbroth dilution technique. The HPLC-purified fraction showed the MICs ranging from 0.5 to 16 μg/ml for methicillin and vancomycin-resistant Staphylococcus aureus (MVRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) strains. The molecular mass of the antimicrobial compound was determined to be 842.37 Da. The same antimicrobial fraction showed negligible haemolytic activity against human red blood cells even at a concentration as high as 100 μg/ml. Because of its significant antimicrobial activity at low MIC values coupled with its non-haemolytic property, it may prove to be a novel antimicrobial lead molecule.

摘要

在本研究中，枯草芽孢杆菌URID 12.1的无细胞改良胰蛋白胨大豆肉汤（pH 7.4±0.2）对耐多药的金黄色葡萄球菌、表皮葡萄球菌、化脓性链球菌和粪肠球菌菌株显示出显著的抗菌活性。发现部分纯化的抗菌分子对极端的pH值和温度具有抗性，并且对较高浓度的胰蛋白酶和蛋白酶K也具有抗性。抗菌分子通过包括反相高效液相色谱（RP-HPLC）在内的三步法进行纯化。使用微量肉汤稀释技术测定了14种细菌的最低抑菌浓度（MIC）值。HPLC纯化的级分对耐甲氧西林和万古霉素的金黄色葡萄球菌（MVRSA）以及耐甲氧西林的表皮葡萄球菌（MRSE）菌株的MIC范围为0.5至16μg/ml。抗菌化合物的分子量测定为842.37Da。即使在高达100μg/ml的浓度下，相同的抗菌级分对人红细胞的溶血活性也可忽略不计。由于其在低MIC值下具有显著的抗菌活性以及其非溶血特性，它可能被证明是一种新型的抗菌先导分子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/84d98f48a38e/fmicb-06-01335-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/72f375544bad/fmicb-06-01335-g0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/6487b1d3b6fe/fmicb-06-01335-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/5957c3638fc5/fmicb-06-01335-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/84d98f48a38e/fmicb-06-01335-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/72f375544bad/fmicb-06-01335-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/d4e3be054c63/fmicb-06-01335-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/fd4b5b293d69/fmicb-06-01335-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/81e641b037f2/fmicb-06-01335-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/6487b1d3b6fe/fmicb-06-01335-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/5957c3638fc5/fmicb-06-01335-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3f9/4678185/84d98f48a38e/fmicb-06-01335-g0007.jpg

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