Singhal Prabhat K, Sassi Slim, Lan Lan, Au Patrick, Halvorsen Stefan C, Fukumura Dai, Jain Rakesh K, Seed Brian
Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114; Department of Genetics, Harvard Medical School, Boston, MA 02115;
Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114; Department of Orthopedics, Harvard Medical School, Boston, MA 02115;
Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):122-7. doi: 10.1073/pnas.1522401112. Epub 2015 Dec 22.
Analysis of embryonic fibroblasts from GFP reporter mice indicates that the fibroblast cell type harbors a large collection of developmentally and phenotypically heterogeneous subtypes. Some of these cells exhibit multipotency, whereas others do not. Multiparameter flow cytometry analysis shows that a large number of distinct populations of fibroblast-like cells can be found in cultures initiated from different embryonic organs, and cells sorted according to their surface phenotype typically retain their characteristics on continued propagation in culture. Similarly, surface phenotypes of individual cloned fibroblast-like cells exhibit significant variation. The fibroblast cell class appears to contain a very large number of denumerable subtypes.
对来自绿色荧光蛋白（GFP）报告基因小鼠的胚胎成纤维细胞进行分析表明，成纤维细胞类型包含大量发育和表型上异质的亚型。其中一些细胞表现出多能性，而另一些则没有。多参数流式细胞术分析显示，从不同胚胎器官起始的培养物中可发现大量不同的成纤维细胞样细胞群体，根据其表面表型分选的细胞在继续传代培养时通常会保留其特征。同样，单个克隆的成纤维细胞样细胞的表面表型也表现出显著差异。成纤维细胞类别似乎包含非常大量可数的亚型。
