Lobb D K, Kobrin M S, Kudlow J E, Dorrington J H
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
Biol Reprod. 1989 May;40(5):1087-93. doi: 10.1095/biolreprod40.5.1087.
The pituitary gonadotropins and gonadal steroids are required for normal follicular growth and development but neither has been shown to act directly as a granulosa cell mitogen in vitro. A number of polypeptide growth factors, however, are known to have pronounced mitogenic effects on the cells of the follicle. We have localized transforming growth factor-alpha (TGF-alpha), a potent mitogen, in bovine thecal cells via immunoperoxidase staining using a monoclonal antibody for TGF-alpha that does not cross-react with epidermal growth factor. TGF-alpha staining is most intense in the theca of follicles at the discrete physiological stages known to show rapid granulosa cell growth (small follicles of 0.7-2.0 mm diameter). Staining intensity for TGF-alpha declines in large preovulatory follicles, coincident with the known decline in granulosa cell mitosis. These studies provide further evidence for paracrine interactions in the ovary and show that TGF-alpha may play an important role in the regulation of follicular development in the adult bovine ovary.
垂体促性腺激素和性腺类固醇是卵泡正常生长和发育所必需的，但在体外尚未显示它们能直接作为颗粒细胞有丝分裂原发挥作用。然而，已知一些多肽生长因子对卵泡细胞有显著的促有丝分裂作用。我们使用一种不与表皮生长因子发生交叉反应的抗转化生长因子-α（TGF-α）单克隆抗体，通过免疫过氧化物酶染色法，在牛的卵泡膜细胞中定位了一种有效的有丝分裂原——转化生长因子-α。在已知颗粒细胞快速生长的离散生理阶段（直径为0.7 - 2.0毫米的小卵泡），TGF-α染色在卵泡膜中最为强烈。在排卵前的大卵泡中，TGF-α的染色强度下降，这与已知的颗粒细胞有丝分裂减少相一致。这些研究为卵巢中的旁分泌相互作用提供了进一步的证据，并表明TGF-α可能在成年牛卵巢卵泡发育的调节中发挥重要作用。
