Wang Yanyan, Surzenko Natalia, Friday Walter B, Zeisel Steven H
*Nutrition Research Institute, University of North Carolina at Chapel Hill, Kannapolis, North Carolina, USA, Department of Medical Genetics, Third Military Medical University, Chongqing, China; and Department of Nutrition, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
*Nutrition Research Institute, University of North Carolina at Chapel Hill, Kannapolis, North Carolina, USA, Department of Medical Genetics, Third Military Medical University, Chongqing, China; and Department of Nutrition, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
FASEB J. 2016 Apr;30(4):1566-78. doi: 10.1096/fj.15-282426. Epub 2015 Dec 23.
Maternal diets low in choline, an essential nutrient, increase the risk of neural tube defects and lead to low performance on cognitive tests in children. However, the consequences of maternal dietary choline deficiency for the development and structural organization of the cerebral cortex remain unknown. In this study, we fed mouse dams either control (CT) or low-choline (LC) diets and investigated the effects of choline on cortical development in the offspring. As a result of a low choline supply between embryonic day (E)11 and E17 of gestation, the number of 2 types of cortical neural progenitor cells (NPCs)-radial glial cells and intermediate progenitor cells-was reduced in fetal brains (P< 0.01). Furthermore, the number of upper layer cortical neurons was decreased in the offspring of dams fed an LC diet at both E17 (P< 0.001) and 4 mo of age (P< 0.001). These effects of LC maternal diet were mediated by a decrease in epidermal growth factor receptor (EGFR) signaling in NPCs related to the disruption of EGFR posttranscriptional regulation. Our findings describe a novel mechanism whereby low maternal dietary intake of choline alters brain development.-Wang, Y., Surzenko, N., Friday, W. B., Zeisel, S. H. Maternal dietary intake of choline in mice regulates development of the cerebral cortex in the offspring.
胆碱是一种必需营养素，孕期母亲饮食中胆碱含量低会增加胎儿神经管缺陷的风险，并导致儿童认知测试表现不佳。然而，孕期母亲饮食中胆碱缺乏对大脑皮层发育和结构组织的影响尚不清楚。在本研究中，我们给孕鼠喂食对照（CT）或低胆碱（LC）饮食，并研究胆碱对后代皮质发育的影响。由于在妊娠第11天（E）至第17天期间胆碱供应不足，胎脑中两种皮质神经祖细胞（NPC）——放射状胶质细胞和中间祖细胞的数量减少（P<0.01）。此外，在E17（P<0.001）和4月龄（P<0.001）时，喂食LC饮食的孕鼠后代中上层皮质神经元的数量均减少。孕期母亲LC饮食的这些影响是由NPC中表皮生长因子受体（EGFR）信号减少介导的，这与EGFR转录后调控的破坏有关。我们的研究结果描述了一种新机制，即孕期母亲饮食中胆碱摄入量低会改变大脑发育。——王Y、苏尔岑科N、弗里迪W.B.、蔡塞尔S.H. 小鼠孕期母亲饮食中胆碱摄入量调节后代大脑皮层发育。
