Wang Qiuju, Ju Xue, Chen Yuke, Dong Xiaoqing, Luo Sha, Liu Hongjian, Zhang Dongming
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Fishery Technical Extension Station of Jilin Province, Changchun, 130012, China.
Fish Physiol Biochem. 2016 Jun;42(3):845-57. doi: 10.1007/s10695-015-0179-x. Epub 2015 Dec 23.
This study was designed in vitro to investigate the effects of L-carnitine against H2O2-induced oxidative stress in a grass carp (Ctenopharyngodon idellus) ovary cell line (GCO). GCO cells were pre-treated with different concentrations of L-carnitine, followed by incubation with 2.5 mM H2O2 for 1 h to induce oxidative damage. The results indicated that adding L-carnitine at concentrations of 0.01-1 mM into the medium for 12 h significantly increased cell viability. Pre-treatment with L-carnitine at concentrations of 0.1-5 mM for 12 h significantly inhibited 2.5 mM H2O2-induced cell viability loss. The significant decreases in the level of reactive oxygen species and cell apoptosis were observed in 0.5 mM L-carnitine group compared to the H2O2 group. Malondialdehyde values of all of the L-carnitine groups were significantly lower than those of the H2O2 group, while total glutathione levels of all of the L-carnitine groups were significantly higher than of the H2O2 group. The activity of antioxidant enzymes, such as total superoxide dismutase (0.1 and 0.5 mM L-carnitine), catalase (0.5 mM L-carnitine) and γ-glutamyl cysteine synthetase (0.5 and 1 mM L-carnitine), was significantly increased. In addition, pre-treatment of L-carnitine in GCO cells exposed to 2.5 mM H2O2 significantly increased the mRNA expression of copper, zinc superoxide dismutase, catalase (0.5 mM L-carnitine), glutamate cysteine ligase catalytic subunit (0.1-1 mM) and glutathione peroxidase (0.1 mM L-carnitine). In conclusion, L-carnitine promotes GCO cell growth and improves antioxidant function, it plays a protective role against oxidative stress induced by H2O2 in GCO cells, and the appropriate supplemental amount of L-carnitine is 0.1-1 mM.
本研究在体外进行，旨在探讨左旋肉碱对过氧化氢（H2O2）诱导的草鱼（Ctenopharyngodon idellus）卵巢细胞系（GCO）氧化应激的影响。GCO细胞先用不同浓度的左旋肉碱预处理，然后与2.5 mM H2O2孵育1小时以诱导氧化损伤。结果表明，在培养基中添加浓度为0.01 - 1 mM的左旋肉碱12小时可显著提高细胞活力。用浓度为0.1 - 5 mM的左旋肉碱预处理12小时可显著抑制2.5 mM H2O2诱导的细胞活力丧失。与H2O2组相比，0.5 mM左旋肉碱组的活性氧水平和细胞凋亡显著降低。所有左旋肉碱组的丙二醛值均显著低于H2O2组，而所有左旋肉碱组的总谷胱甘肽水平均显著高于H2O2组。抗氧化酶的活性，如总超氧化物歧化酶（0.1和0.5 mM左旋肉碱）、过氧化氢酶（0.5 mM左旋肉碱）和γ-谷氨酰半胱氨酸合成酶（0.5和1 mM左旋肉碱）显著增加。此外，在暴露于2.5 mM H2O2的GCO细胞中，左旋肉碱预处理显著增加了铜锌超氧化物歧化酶、过氧化氢酶（0.5 mM左旋肉碱）、谷氨酸半胱氨酸连接酶催化亚基（0.1 - 1 mM）和谷胱甘肽过氧化物酶（0.1 mM左旋肉碱）的mRNA表达。总之，左旋肉碱促进GCO细胞生长并改善抗氧化功能，它对GCO细胞中H2O2诱导的氧化应激起到保护作用，左旋肉碱的适宜补充量为0.1 - 1 mM。
