Center for Surgery, Department of Orthopedics and Trauma Surgery, Medical Center-University of Freiburg, Hugstetter str. 55, Freiburg D-79106, Germany.
Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering-IMTEK, University of Freiburg, Georges-Koehler-Allee 103, Freiburg D-79110, Germany.
J Funct Biomater. 2015 Dec 21;6(4):1085-98. doi: 10.3390/jfb6041085.
The aim of this study was the development of a process for filling the pores of a β-tricalcium phosphate ceramic with interconnected porosity with an alginate hydrogel. For filling of the ceramics, solutions of alginate hydrogel precursors with suitable viscosity were chosen as determined by rheometry. For loading of the porous ceramics with the gel the samples were placed at the flow chamber and sealed with silicone seals. By using a vacuum induced directional flow, the samples were loaded with alginate solutions. The loading success was controlled by ESEM and fluorescence imaging using a fluorescent dye (FITC) for staining of the gel. After loading of the pores, the alginate is transformed into a hydrogel through crosslinking with CaCl₂ solution. The biocompatibility of the obtained composite material was tested with a live dead cell staining by using MG-63 Cells. The loading procedure via vacuum assisted directional flow allowed complete filling of the pores of the ceramics within a few minutes (10 ± 3 min) while loading through simple immersion into the polymer solution or through a conventional vacuum method only gave incomplete filling.
本研究的目的是开发一种用藻酸盐水凝胶填充具有互连通孔的 β-磷酸三钙陶瓷的方法。为了填充陶瓷，选择了通过流变学确定的具有合适粘度的藻酸盐水凝胶前体溶液。为了将凝胶装入多孔陶瓷中，将样品放置在流动室中并用硅橡胶密封。通过使用真空诱导的定向流动，将样品用藻酸盐溶液加载。通过使用荧光染料（FITC）对凝胶进行染色，通过 ESEM 和荧光成像来控制加载的成功。在加载孔隙后，藻酸盐通过与 CaCl₂溶液交联转化为水凝胶。通过使用 MG-63 细胞进行活死细胞染色来测试获得的复合材料的生物相容性。通过真空辅助定向流动的加载程序可以在几分钟内（10±3 分钟）完全填充陶瓷的孔隙，而通过简单浸入聚合物溶液或通过常规的真空方法进行加载只能实现不完全填充。
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