基于果胶-金纳米复合材料的铜绿假单胞菌特异性和选择性电化学免疫分析。
Specific and selective electrochemical immunoassay for Pseudomonas aeruginosa based on pectin-gold nano composite.
机构信息
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.
Department of Natural Product Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, India.
出版信息
Biosens Bioelectron. 2016 May 15;79:121-9. doi: 10.1016/j.bios.2015.12.006. Epub 2015 Dec 9.
In this report, we have successfully fabricated an immunosensor for detection of Pseudomonas aeruginosa in water. The monoclonal antibody was immobilized on the surface modified with CCLP (Calcium Cross-Linked Pectin)-Au NPs (gold nanoparticles)/Glassy Carbon Electrode. The building of the immunosensor was evaluated in each step by cyclic voltammetry (CV) and impedance spectroscopy (EIS). The electrochemical detection was done based on the anti rabbit IgG HRP (Horseradish Peroxidase) which binds to the immune complex and the response was observed using Hydro Quininone (HQ) and Hydrogen peroxide (H2O2) in PB (Phosphate Buffer) electrolyte. From the results, the sensitivity range is from 10(1) to 10(7)CFU/ml and LOD is calculated as 9×10(2)CFU/ml. The developed immunosensor also have high selectivity, stability, reproducibility and reusability.
在本报告中，我们成功地制备了一种用于检测水中铜绿假单胞菌的免疫传感器。该单克隆抗体固定在经 CCLP（钙交联果胶）-Au NPs（金纳米粒子）/玻碳电极修饰的表面上。通过循环伏安法（CV）和阻抗谱（EIS）对免疫传感器的构建进行了每一步的评估。电化学检测基于与免疫复合物结合的抗兔 IgG HRP（辣根过氧化物酶）进行，并使用 HQ（Hydro Quininone）和 PB（磷酸盐缓冲液）电解质中的 H2O2（过氧化氢）观察到响应。结果表明，灵敏度范围为 10(1)至 10(7)CFU/ml，LOD 计算为 9×10(2)CFU/ml。所开发的免疫传感器还具有高选择性、稳定性、重现性和可重复性。
Zotero 插件