Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences, Beijing 100083, PR China; School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences, Beijing 100083, PR China.
Biosens Bioelectron. 2017 Jun 15;92:33-39. doi: 10.1016/j.bios.2017.01.065. Epub 2017 Jan 31.
Recently early diagnosis of squamous cell carcinoma antigen (SCCA) as a tumor maker of various cancers has increasingly attracted a lot of attention with heightening of incidence rate of cancer. The SCCA with low concentration in human serum should be diluted before detecting. Thus, an immunoassay with high sensitivity is significant for early detecting SCCA. Therefore, a nonenzymatic sandwich-type electrochemical immunosensor herein was conducted to quantitative detection of squamous cell carcinoma antigen (SCCA). The amino functionalized cobaltosic oxide @ ceric dioxide nanocubes with core-shell morphology were prepared to combine sea-urchin like gold @ platinum nanoparticles (CoO@CeO-Au@Pt), and used as labels to conjugate with secondary antibodies for signal amplification. Due to the synergetic effect, excellent electrochemical property and superior auxiliary catalytic activity of CoO@CeO-Au@Pt, high electrocatalytic current responses toward the reduction of hydrogen peroxide (HO) were achieved. Besides, the electrodeposited gold nanoparticles (D-Au NPs) which were modified on glassy carbon electrodes (GCE) were used as antibodies carriers and sensing platforms. With the well cooperation of CoO@CeO-Au@Pt and D-Au NPs, a broad linear range from 100fg/mL to 80ng/mL with a low detection limit of 33 fg/mL for detecting SCCA was achieved. In addition, the immunosensor displayed with good reproducibility, high selectivity and stability. The results are satisfactory when the proposed method has been applied to analyze human serum samples, indicating that the potential application is promising in clinical monitoring of tumor markers.
最近，随着癌症发病率的升高，鳞状细胞癌抗原（SCCA）作为各种癌症的肿瘤标志物，早期诊断越来越受到关注。人血清中 SCCA 浓度较低，检测前应进行稀释。因此，对于 SCCA 的早期检测，高灵敏度的免疫分析方法具有重要意义。因此，本文构建了一种非酶免的三明治型电化学免疫传感器，用于定量检测鳞状细胞癌抗原（SCCA）。通过制备具有核壳结构的氨基功能化氧化钴@二氧化铈纳米立方体（CoO@CeO-Au@Pt），将其作为标签与二级抗体结合，用于信号放大。由于协同作用，CoO@CeO-Au@Pt 具有优异的电化学性能和辅助催化活性，对过氧化氢（HO）的还原表现出高电催化电流响应。此外，在玻碳电极（GCE）上修饰的电沉积金纳米颗粒（D-Au NPs）被用作抗体载体和传感平台。在 CoO@CeO-Au@Pt 和 D-Au NPs 的协同作用下，实现了 SCCA 从 100fg/mL 到 80ng/mL 的宽线性范围，检测限低至 33 fg/mL。此外，该免疫传感器表现出良好的重现性、高选择性和稳定性。当将所提出的方法应用于分析人血清样品时，结果令人满意，表明该方法在肿瘤标志物的临床监测中有很好的应用前景。
