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新种阿米尔斯脱硫菌,一种从酸性河流沉积物中分离出的新型耐酸硫呼吸细菌。

Desulfurella amilsii sp. nov., a novel acidotolerant sulfur-respiring bacterium isolated from acidic river sediments.

作者信息

Florentino Anna P, Brienza Claudio, Stams Alfons J M, Sánchez-Andrea Irene

机构信息

Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.

Sub-department of Environmental Technology, Wageningen University, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.

出版信息

Int J Syst Evol Microbiol. 2016 Mar;66(3):1249-1253. doi: 10.1099/ijsem.0.000866. Epub 2015 Dec 23.

Abstract

A novel acidotolerant and moderately thermophilic sulfur-reducing bacterium was isolated from sediments of the Tinto River (Spain), an extremely acidic environment. Strain TR1T stained Gram-negative, and was obligately anaerobic, non-spore-forming and motile. Cells were short rods (1.5-2 × 0.5-0.7 μm), appearing singly or in pairs. Strain TR1T was catalase-negative and slightly oxidase-positive. Urease activity and indole formation were absent, but gelatin hydrolysis was present. Growth was observed at 20-52 °C with an optimum close to 50 °C, and a pH range of 3-7 with optimum between pH 6 and 6.5. Yeast extract was essential for growth, but extra vitamins were not required. In the presence of sulfur, strain TR1T grew with acetate, formate, lactate, pyruvate, stearate, arginine and H2/CO2. All substrates were completely oxidized and H2S and CO2 were the only metabolic products detected. Besides elemental sulfur, thiosulfate was used as an electron acceptor. The isolate also grew by disproportionation of elemental sulfur. The predominant cellular fatty acids were saturated components: C16 : 0, anteiso-C17 : 0 and C18 : 0. The only quinone component detected was menaquinone MK-7(H2). The G+C content of the genomic DNA was 34 mol%. The isolate is affiliated to the genus Desulfurella of the class Deltaproteobacteria, sharing 97 % 16S rRNA gene sequence similarity with the four species described in the genus Desulfurella. Considering the distinct physiological and phylogenetic characteristics, strain TR1T represents a novel species within the genus Desulfurella, for which the name Desulfurella amilsii sp. nov. is proposed. The type strain is TR1T ( = DSM 29984T = JCM 30680T).

摘要

从西班牙廷托河(一个极端酸性环境)的沉积物中分离出一种新型的耐酸且嗜温适度的硫还原菌。菌株TR1T革兰氏染色阴性,专性厌氧,不形成芽孢且具运动性。细胞为短杆状(1.5 - 2×0.5 - 0.7μm),单个或成对出现。菌株TR1T过氧化氢酶阴性,氧化酶弱阳性。无脲酶活性和吲哚生成,但有明胶水解。在20 - 52°C可生长,最适温度接近50°C,pH范围为3 - 7,最适pH在6至6.5之间。酵母提取物对生长至关重要,但不需要额外的维生素。在有硫存在的情况下,菌株TR1T可利用乙酸盐、甲酸盐、乳酸盐、丙酮酸盐、硬脂酸盐、精氨酸以及H2/CO2生长。所有底物均被完全氧化,仅检测到H2S和CO2作为代谢产物。除了元素硫,硫代硫酸盐用作电子受体。该分离物也可通过元素硫的歧化作用生长。主要的细胞脂肪酸为饱和成分:C16 : 0、anteiso - C17 : 0和C18 : 0。检测到的唯一醌类成分是甲基萘醌MK - 7(H2)。基因组DNA的G + C含量为34 mol%。该分离物隶属于δ-变形菌纲脱硫弧菌属,与脱硫弧菌属中已描述的四个物种16S rRNA基因序列相似度为97 %。鉴于其独特的生理和系统发育特征,菌株TR1T代表脱硫弧菌属内的一个新物种,为此提议命名为Desulfurella amilsii sp. nov.。模式菌株为TR1T(= DSM 29984T = JCM 30680T)。

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