Vulpe Raluca, Popa Marcel, Picton Luc, Balan Vera, Dulong Virginie, Butnaru Maria, Verestiuc Liliana
"Gheorghe Asachi" Technical University, Faculty of Chemical Engineering and Environmental Protection, Department of Natural and Synthetic Polymers, 73 Prof. dr. docent Dimitrie Mangeron Street, 700050 Iasi, Romania; Université de Rouen, Laboratoire Polymères Biopolymères Surfaces, F-76821 Mont Saint Aignan, France.
"Gheorghe Asachi" Technical University, Faculty of Chemical Engineering and Environmental Protection, Department of Natural and Synthetic Polymers, 73 Prof. dr. docent Dimitrie Mangeron Street, 700050 Iasi, Romania; Academy of Romanian Scientist, 54 Splaiul Indenpendentei Street, 050094 Bucuresti, Romania.
Int J Biol Macromol. 2016 Mar;84:174-81. doi: 10.1016/j.ijbiomac.2015.12.019. Epub 2015 Dec 15.
Zero-length crosslinked hydrogels have been synthesized by covalent linking of three natural polymers (collagen, hyaluronic acid and sericin), in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. The hydrogels have been investigated by FT-IR spectroscopy, microcalorimetry, in vitro swelling, enzymatic degradation, and in vitro cell viability studies. The obtained crosslinked hydrogels showed a macroporous structure, high swelling degree and in vitro enzymatic resistance compared to uncrosslinked collagen. The in vitro cell viability studies performed on normal human dermal fibroblasts assessed the sericin proliferation properties indicating a potential use of the hydrogels based on collagen, hyaluronic acid and sericin in skin tissue engineering.
通过在1-乙基-3-(3-二甲基氨基丙基)碳二亚胺和N-羟基琥珀酰亚胺存在的情况下，将三种天然聚合物（胶原蛋白、透明质酸和丝胶蛋白）共价连接，合成了零长度交联水凝胶。通过傅里叶变换红外光谱、微量热法、体外溶胀、酶降解和体外细胞活力研究对水凝胶进行了研究。与未交联的胶原蛋白相比，所得的交联水凝胶显示出大孔结构、高溶胀度和体外酶抗性。对正常人皮肤成纤维细胞进行的体外细胞活力研究评估了丝胶蛋白的增殖特性，表明基于胶原蛋白、透明质酸和丝胶蛋白的水凝胶在皮肤组织工程中有潜在用途。
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