Tincu Iurciuc Camelia Elena, Daraba Oana Maria, Jérôme Christine, Popa Marcel, Ochiuz Lăcrămioara
Department of Natural and Synthetic Polymers, Faculty of Chemical Engineering and Protection of the Environment, "Gheorghe Asachi" Technical University, 73 Prof. Dr. Docent Dimitrie Mangeron Street, 700050 Iasi, Romania.
Department of Pharmaceutical Technology, Faculty of Pharmacy, "Grigore T. Popa" University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, Romania.
Polymers (Basel). 2024 Jun 8;16(12):1631. doi: 10.3390/polym16121631.
Bovine serum albumin (BSA) hydrogels are non-immunogenic, low-cost, biocompatible, and biodegradable. In order to avoid toxic cross-linking agents, gellan was oxidized with NaIO to obtain new functional groups like dialdehydes for protein-based hydrogel cross-linking. The formed dialdehyde groups were highlighted with FT-IR and NMR spectroscopy. This paper aims to investigate hydrogel films for biomedical applications obtained by cross-linking BSA with oxidized gellan (OxG) containing immobilized β-cyclodextrin-curcumin inclusion complex (β-CD-Curc) The β-CD-Curc improved the bioavailability and solubility of Curc and was prepared at a molar ratio of 2:1. The film's structure and morphology were evaluated using FT-IR spectroscopy and SEM. The swelling degree (Q%) values of hydrogel films depend on hydrophilicity and pH, with higher values at pH = 7.4. Additionally, the conversion index of -NH groups into Schiff bases increases with an increase in OxG amount. The polymeric matrix provides protection for Curc, is non-cytotoxic, and enhances antioxidant activity. At pH = 5.5, the skin permeability and release efficiency of encapsulated curcumin were higher than at pH = 7.4 because of the interaction of free aldehyde and carboxylic groups from hydrogels with amine groups from proteins present in the skin membrane, resulting in a better film adhesion and more efficient curcumin release.
牛血清白蛋白（BSA）水凝胶具有非免疫原性、低成本、生物相容性和可生物降解性。为了避免使用有毒的交联剂，用naio氧化结冷胶以获得新的官能团，如用于基于蛋白质的水凝胶交联的二醛。通过傅里叶变换红外光谱（FT-IR）和核磁共振光谱（NMR）突出显示形成的二醛基团。本文旨在研究通过将BSA与含有固定化β-环糊精-姜黄素包合物（β-CD-Curc）的氧化结冷胶（OxG）交联而获得的用于生物医学应用的水凝胶薄膜。β-CD-Curc提高了姜黄素的生物利用度和溶解度，其制备的摩尔比为2:1。使用FT-IR光谱和扫描电子显微镜（SEM）评估薄膜的结构和形态。水凝胶薄膜的溶胀度（Q%）值取决于亲水性和pH值，在pH = 7.4时具有更高的值。此外，-NH基团转化为席夫碱的转化指数随着OxG量的增加而增加。聚合物基质为姜黄素提供保护，无细胞毒性，并增强抗氧化活性。在pH = 5.5时，由于水凝胶中的游离醛和羧基与皮肤膜中存在的蛋白质的胺基相互作用，包封的姜黄素的皮肤渗透性和释放效率高于pH = 7.4时，从而导致更好的薄膜粘附和更有效的姜黄素释放。
