Vollrath D, Davis R W
Department of Biochemistry, Stanford University School of Medicine, CA 94305.
Nucleic Acids Res. 1987 Oct 12;15(19):7865-76. doi: 10.1093/nar/15.19.7865.
Excellent resolution of chromosomal DNA molecules from Saccharomyces cerevisiae, Candida albicans and Schizosaccharomyces pombe has been obtained using alternating contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The largest of these molecules is greater than 5 Mb in size and is resolved after 130 hours in a 0.6% agarose gel at a field strength of 1.3 V/cm and a switching interval of 1 hour. Separation of concatamers of phage lambda DNA reveals four regions of resolution in alternating CHEF gel electrophoresis. There are two regions of good resolution in which mobility approximates a linear function of molecular weight. These are separated by a region of lower resolution and bounded at high molecular weights by a region of little or no resolution. The four regions are of practical and possibly theoretical importance.
使用交变轮廓钳位均匀电场（CHEF）凝胶电泳已获得来自酿酒酵母、白色念珠菌和粟酒裂殖酵母的染色体DNA分子的出色分辨率。这些分子中最大的尺寸大于5 Mb，在0.6%琼脂糖凝胶中，场强为1.3 V/cm且切换间隔为1小时的条件下，经过130小时后得以分离。噬菌体λ DNA串联体的分离在交变CHEF凝胶电泳中显示出四个分辨率区域。有两个分辨率良好的区域，其中迁移率近似于分子量的线性函数。这两个区域被一个分辨率较低的区域隔开，在高分子量区域则由一个几乎没有或没有分辨率的区域界定。这四个区域具有实际和可能的理论重要性。
