Samarghandian Saeed, Azimi-Nezhad Mohsen, Farkhondeh Tahereh, Samini Fariborz
Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran.
Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Biomed Pharmacother. 2017 Mar;87:223-229. doi: 10.1016/j.biopha.2016.12.105. Epub 2017 Jan 3.
Restraint stress has been indicated to induce oxidative damage in tissues. Several investigations have reported that curcumin (CUR) may have a protective effect against oxidative stress. The present study was designed to investigate the protective effects of CUR on restraint stress induced oxidative stress damage in the brain, liver and kidneys. For chronic restraint stress, rats were kept in the restrainers for 1h every day, for 21 consecutive days. The animals received systemic administrations of CUR daily for 21days. In order to evaluate the changes of the oxidative stress parameters following restraint stress, the levels of malondialdehyde (MDA), reduced glutathione (GSH), as well as antioxidant enzyme activities superoxide dismutase (SOD) glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) were measured in the brain, liver and kidney of rats after the end of restraint stress. The restraint stress significantly increased MDA level, but decreased the level of GSH and activists of SOD, GPx, GR, and CAT the brain, liver and kidney of rats in comparison to the normal rats (P<0.001). Intraperitoneal administration of CUR significantly attenuated oxidative stress and lipid peroxidation, prevented apoptosis, and increased antioxidant defense mechanism activity in the tissues versus the control group (P<0.05). This study shows that CUR can prevent restraint stress-induced oxidative damage in the brain, liver and kidney of rats and propose that CUR may be useful agents against oxidative stress in the tissues.
束缚应激已被证实会诱导组织中的氧化损伤。多项研究报告称，姜黄素（CUR）可能对氧化应激具有保护作用。本研究旨在探讨CUR对束缚应激诱导的大鼠脑、肝和肾氧化应激损伤的保护作用。对于慢性束缚应激，大鼠每天在束缚器中放置1小时，连续21天。动物连续21天每天接受CUR的全身给药。为了评估束缚应激后氧化应激参数的变化，在束缚应激结束后，测量大鼠脑、肝和肾中丙二醛（MDA）、还原型谷胱甘肽（GSH）的水平以及抗氧化酶超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和过氧化氢酶（CAT）的活性。与正常大鼠相比，束缚应激显著提高了大鼠脑、肝和肾中MDA水平，但降低了GSH水平以及SOD、GPx、GR和CAT的活性（P<0.001）。与对照组相比，腹腔注射CUR可显著减轻氧化应激和脂质过氧化，预防细胞凋亡，并提高组织中的抗氧化防御机制活性（P<0.05）。本研究表明，CUR可预防束缚应激诱导的大鼠脑、肝和肾氧化损伤，并表明CUR可能是对抗组织氧化应激的有用药物。
