Abarikwu Sunny O, Simple Godwin, Onuoha Chimezie Samuel
Department of Biochemistry, University of Port Harcourt, Choba, Nigeria.
Indian J Clin Biochem. 2020 Jan;35(1):20-31. doi: 10.1007/s12291-018-0788-0. Epub 2018 Sep 15.
The antioxidant protective effects of gallic acid (GAL) and quercetin (QUE) against oxidative stress induced by di-butyl phthalate (DnBP) in the liver and testis of rats were evaluated in this study. Adult albino Wistar rats (180-225 g) were treated with QUE or GAL (50 mg/kg) alone or in combination with DnBP (1 mL/kg) for 15 days. After treatment, tissue samples were taken for determination of glutathione and malondialdehyde levels, and superoxide dismutase and catalase activities. Serial sections of the testis and liver were stained with haematoxylin and eosin for microscopy and seminiferous tubular morphometry. As expected, DnBP induced oxidative stress was evident by increased malondialdehyde level in both organs. Co-treatment with GAL or QUE reversed the malondialdehyde by 45.42, 37.44 and 37.57%, 23.32% and catalase by 52.21, 70.15 and 85%, 38.14% in the testis and liver respectively whereas superoxide dismutase activity and glutathione level were differently modulated parallel to histopathological improvement in both tissues. The seminiferous tubular diameter, epithelial height, epithelial germ cell count and tubular length were significantly decreased by 11.09, 51.91, 40.65 and 11.10% respectively versus control values after DnBP treatments and were attenuated on co-treatment with GAL or QUE. Co-treatment with GAL afforded better protective effects in both tissues but QUE treatment alone appeared more effective than GAL on the investigated morphometric data. It seems likely that GAL or QUE prevented the tissue damage but the antioxidant profiles of the liver and testis are different in response to the oxidative stress.
本研究评估了没食子酸（GAL）和槲皮素（QUE）对大鼠肝脏和睾丸中邻苯二甲酸二丁酯（DnBP）诱导的氧化应激的抗氧化保护作用。成年白化Wistar大鼠（180 - 225克）单独用QUE或GAL（50毫克/千克）处理，或与DnBP（1毫升/千克）联合处理15天。处理后，采集组织样本以测定谷胱甘肽和丙二醛水平以及超氧化物歧化酶和过氧化氢酶活性。睾丸和肝脏的连续切片用苏木精和伊红染色以进行显微镜检查和生精小管形态测量。正如预期的那样，DnBP诱导的氧化应激在两个器官中均表现为丙二醛水平升高。与GAL或QUE共同处理分别使睾丸和肝脏中的丙二醛水平降低了45.42%、37.44%和37.57%、23.32%，使过氧化氢酶活性分别提高了52.21%、70.15%和85%、38.14%，而超氧化物歧化酶活性和谷胱甘肽水平则随着两个组织的组织病理学改善而受到不同程度的调节。与对照值相比，DnBP处理后生精小管直径、上皮高度、上皮生殖细胞计数和小管长度分别显著降低了11.09%、51.91%、40.65%和11.10%，而与GAL或QUE共同处理后这些指标有所减轻。与GAL共同处理在两个组织中均提供了更好的保护作用，但单独使用QUE处理在所研究的形态测量数据方面似乎比GAL更有效。GAL或QUE似乎可以预防组织损伤，但肝脏和睾丸对氧化应激的抗氧化谱不同。
