Ciocca L, Lesci I G, Mezini O, Parrilli A, Ragazzini S, Rinnovati R, Romagnoli N, Roveri N, Scotti R
Department of Biomedical and Neuromotor Science, Section of Prosthodontics, Alma Mater Studiorum University of Bologna, 40125, Bologna, Italy.
Laboratory for Environment Biotechnology Structural engineering and Chemistry, LEBSC s.r.l. Bologna, Italy.
J Biomed Mater Res B Appl Biomater. 2017 May;105(4):723-734. doi: 10.1002/jbm.b.33597. Epub 2015 Dec 28.
Three-dimension (3D) scaffolds for bone tissue regeneration were produced combining three different phases: nanometric hydroxyapatite (HA) was synthesized by precipitation method and the crystals nucleation took place directly within collagen fibrils following a biologically inspired mineralization process; polycaprolactone was employed to give the material a 3D structure. The chemico-physical analysis carried out to test the material's properties and composition revealed a high similarity in composition and morphology with biologically mineralized collagen fibrils and a scaffold degradation pattern suitable for physiological processes. The micro- computerized tomography (micro-CT) showed 53.53% porosity and a 97.86% mean interconnected pores. Computer-aided design and computer-aided manufacturing (CAD-CAM) technology was used for molding the scaffold's volume (design/shape) and for guiding the surgical procedure (cutting guides). The custom made scaffolds were implanted in sheep mandible using prototyped surgical guides and customized bone plates. After three months healing, scanning electron microscopy (SEM) analysis of the explanted scaffold revealed a massive cell seeding of the scaffold, with cell infiltration within the scaffold's interconnected pores. The micro-CT of the explanted construct showed a good match between the scaffold and the adjacent host's bone, to shield the implant primary stability. Histology confirmed cell penetration and widely documented neoangiogenesis within the entire scaffold's volume. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 723-734, 2017.
通过结合三个不同阶段制备了用于骨组织再生的三维（3D）支架：采用沉淀法合成纳米级羟基磷灰石（HA），并且按照生物启发矿化过程，晶体在胶原纤维内直接成核；使用聚己内酯赋予材料三维结构。为测试材料的性能和组成而进行的化学物理分析表明，其在组成和形态上与生物矿化胶原纤维高度相似，并且具有适合生理过程的支架降解模式。微型计算机断层扫描（micro-CT）显示孔隙率为53.53%，平均连通孔隙率为97.86%。利用计算机辅助设计和计算机辅助制造（CAD-CAM）技术来塑造支架的体积（设计/形状）并指导手术过程（切割导向）。使用原型手术导向器和定制骨板将定制的支架植入绵羊下颌骨。经过三个月的愈合后，对取出的支架进行扫描电子显微镜（SEM）分析，结果显示支架上大量细胞着床，细胞浸润到支架的连通孔隙内。取出的构建体的micro-CT显示支架与相邻宿主骨之间匹配良好，以确保植入物的初始稳定性。组织学证实细胞已穿透，并广泛记录了整个支架体积内的新生血管形成。©2016威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B：723 - 734，2017年。
