Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.
Department of Very Important People, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.
Int J Mol Med. 2016 Feb;37(2):493-500. doi: 10.3892/ijmm.2015.2438. Epub 2015 Dec 18.
Concentrated growth factor (CGF) is a newly generated complex that comprises a fibrin matrix incorporating growth factors and plasmatic and leukocyte cytokines. It has been widely used in bone regenerative medicine. However, the effect of CGF on peripheral nerve regeneration had not been previously investigated. The aim of the present study was to evaluate the possibility of using CGF for nerve regeneration by i) investigating the effect of CGF on the proliferation of Schwann cells (SCs) and secretion of neurotrophic factors nerve growth factor (NGF) and glial cell line‑derived neurotrophic factor (GDNF) in vitro; and ii) analyzing the effect of CGF on functional nerve recovery after nerve injury in vivo. CGF was prepared from venous blood taken from rats, and using scanning electron microscopy (SEM) we noted that it featured a fiber‑like appearance with pore size ranging from 0.1 to 1.0 µm. The soluble component of CGF was used to produce conditioned media with which to treat the Schwann cell line. A cell counting kit-8 assay and cell cycle analysis were both used to study the proliferative effect of CGF on SCs. Reverse transcription-quantitative PCR and western blot analysis demonstrated that there was an increase in the mRNA and protein expression of NGF and GDNF, both of which are markers of SC neurotrophic secretion. A model of sciatic nerve crush injury was established for the in vivo experiment, and CGF was found to increase the sciatic functional index (indicative of nerve function). We noted that CGF increased SC proliferation and secretion of neurotrophic factors in vitro, and promoted functional recovery after peripheral nerve injuries in vivo. These results suggest that CGF is a promising candidate biomaterial for peripheral nerve regeneration, and may potentially be utilized to repair nerve injuries.
浓缩生长因子（CGF）是一种新生成的复合物，由包含生长因子、血浆和白细胞细胞因子的纤维基质组成。它已被广泛应用于骨再生医学。然而，CGF 对周围神经再生的影响尚未被研究过。本研究旨在通过以下方法评估 CGF 用于神经再生的可能性：i）研究 CGF 对雪旺细胞（SCs）增殖和神经营养因子神经生长因子（NGF）和胶质细胞源性神经营养因子（GDNF）分泌的体外影响；ii）分析 CGF 对体内神经损伤后功能神经恢复的影响。CGF 是从大鼠静脉血中提取的，通过扫描电子显微镜（SEM）观察到，它具有纤维状外观，孔径大小为 0.1 至 1.0 µm。CGF 的可溶性成分用于制备条件培养基，以治疗雪旺细胞系。使用细胞计数试剂盒-8 检测和细胞周期分析来研究 CGF 对 SC 增殖的影响。逆转录定量 PCR 和 Western blot 分析表明，NGF 和 GDNF 的 mRNA 和蛋白表达均增加，这两者都是 SC 神经营养分泌的标志物。建立了坐骨神经挤压损伤模型进行体内实验，结果发现 CGF 增加了坐骨神经功能指数（神经功能的指标）。我们注意到 CGF 在体外增加了 SC 的增殖和神经营养因子的分泌，并促进了周围神经损伤后的功能恢复。这些结果表明，CGF 是一种有前途的周围神经再生候选生物材料，可能有潜力用于修复神经损伤。
