Olson M V
Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110.
J Chromatogr. 1989 May 26;470(2):377-83. doi: 10.1016/s0021-9673(01)83565-2.
Pulsed-field gel electrophoresis is a method of separating large DNA molecules. The distinctive feature of this method is that the direction of the electric field is changed periodically. During the five years since Schwartz and Cantor introduced this technique, there has been dramatic progress in pulsed-field instrumentation and in associated electrophoretic methods. Progress has been driven by practical experience with little guidance from theory. In this review, the basic phenomenology of pulsed-field gel electrophoresis is summarized and some speculations are advanced about possible molecular mechanisms.
脉冲场凝胶电泳是一种分离大型DNA分子的方法。该方法的独特之处在于电场方向会周期性改变。自从施瓦茨和坎托引入这项技术的五年间，脉冲场仪器及相关电泳方法取得了显著进展。这些进展是由实践经验推动的，理论指导较少。在这篇综述中，总结了脉冲场凝胶电泳的基本现象学，并对可能的分子机制提出了一些推测。
