Gelpí E, Ramis I, Hotter G, Bioque G, Bulbena O, Roselló J
Department of Neurochemistry, CID-CSIC, Barcelona, Spain.
J Chromatogr. 1989 Aug 11;492:223-50. doi: 10.1016/s0378-4347(00)84470-9.
An evaluation of the most recent literature on the determination of eicosanoids by immunoassay methods confirms that owing to the inherent lack of specificity of many of the antibodies used for this purpose, immunological assays (radioimmunoassay or enzyme immunoassay) are often preceded by solid-phase extraction followed by further purification of the antigens of interest by routine reversed-phase high-performance liquid chromatographic methods. In this way the analytical potential of radioimmunoassay is remarkably enhanced and accuracy and precision of the assay are ensured.
对通过免疫测定法测定类二十烷酸的最新文献的评估证实，由于用于此目的的许多抗体固有的特异性不足，免疫测定法（放射免疫测定法或酶免疫测定法）通常先进行固相萃取，然后通过常规反相高效液相色谱法进一步纯化感兴趣的抗原。通过这种方式，放射免疫测定法的分析潜力得到显著提高，并确保了测定的准确性和精密度。
